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Original Research 


Genotyping Diversity of Proteus vulgaris by using RAPD-PCR and Study of Some Factors on the Activity of Bacteria

Lamees A. Abdul Lateef.

Abstract
P. vulgaris causes variety of infection such as, UTIs, wound infections, burn infections, bloodstream infections, and respiratory tract infections, bacteremia and brain abscesses. A total 120 specimen, were collected from clinical samples (urine, vagina, wound, and burn). only 18 isolates of Proteus vulgaris were recovered. The relationship between 18 Proteus vulgaris isolated using RAPD-PCR was performed. The ten primer showed polymorphism among the isolates generating 86 bands, 54 of which were polymorphic with sizes ranging between 150-30000bp. The mean percentage of ten primer 62.79%. Proteus vulgaris isolates identified were screened for biofilm production by TCP method. Among the isolates, 55.55% were biofilm producer and 44.44% were non/weak biofilm producer. Regard the effect of NAC (10%) on biofilm formation was also studied, this study proved that NAC effectively reduced the viability of biofilm formation from (55.55) to (5.55%). On the other hand, the effect of NAC and green tea at concentration (0, 1, 5, 10, and 15 mg/mL) on urease was also investigated, it was state the high concentration of it could cause inhibition to urease production.

Key words: P. vulgaris, cytoplasm, RADP typing.


 
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Pubmed Style

Lamees A. Abdul - Lateef. Genotyping Diversity of Proteus vulgaris by using RAPD-PCR and Study of Some Factors on the Activity of Bacteria. doi:https://doi.org/10.31838/ejmcm.07.02.30


Web Style

Lamees A. Abdul - Lateef. Genotyping Diversity of Proteus vulgaris by using RAPD-PCR and Study of Some Factors on the Activity of Bacteria. http://www.ejmcm.com/?mno=134808 [Access: October 13, 2020]. doi:https://doi.org/10.31838/ejmcm.07.02.30


AMA (American Medical Association) Style

Lamees A. Abdul - Lateef. Genotyping Diversity of Proteus vulgaris by using RAPD-PCR and Study of Some Factors on the Activity of Bacteria. doi:https://doi.org/10.31838/ejmcm.07.02.30



Vancouver/ICMJE Style

Lamees A. Abdul - Lateef. Genotyping Diversity of Proteus vulgaris by using RAPD-PCR and Study of Some Factors on the Activity of Bacteria. doi:https://doi.org/10.31838/ejmcm.07.02.30



Harvard Style

Lamees A. Abdul - Lateef (2020) Genotyping Diversity of Proteus vulgaris by using RAPD-PCR and Study of Some Factors on the Activity of Bacteria. doi:https://doi.org/10.31838/ejmcm.07.02.30



Turabian Style

Lamees A. Abdul - Lateef. 2020. Genotyping Diversity of Proteus vulgaris by using RAPD-PCR and Study of Some Factors on the Activity of Bacteria. doi:https://doi.org/10.31838/ejmcm.07.02.30



Chicago Style

Lamees A. Abdul - Lateef. "Genotyping Diversity of Proteus vulgaris by using RAPD-PCR and Study of Some Factors on the Activity of Bacteria." doi:https://doi.org/10.31838/ejmcm.07.02.30



MLA (The Modern Language Association) Style

Lamees A. Abdul - Lateef. "Genotyping Diversity of Proteus vulgaris by using RAPD-PCR and Study of Some Factors on the Activity of Bacteria." doi:https://doi.org/10.31838/ejmcm.07.02.30



APA (American Psychological Association) Style

Lamees A. Abdul - Lateef (2020) Genotyping Diversity of Proteus vulgaris by using RAPD-PCR and Study of Some Factors on the Activity of Bacteria. doi:https://doi.org/10.31838/ejmcm.07.02.30








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