Aim: The aim of this study to evaluate the microflora in the post-chemotherapy patients of oral cancer. Methods: The present study was conducted in the Department of Dentistry Ananta Institute of Medical Sciences and Research Centre, Tehsil Nathdwara, Distt. Rajsamand, Rajasthan, India, from July 2020 to June 2021. We enrolled 24 patients (15 men and 9 women, aged 20– 55years) with solid malignancy that had no previous adjuvant radiotherapy or recent antimicrobial or antiviral treatment. Sampling was done at the same time of day, approximately 2 h after breakfast. Microorganisms were identified by standard procedures as well as the production of a set of metabolic enzymes (as tested with Rapid ID 32A and Rapid ID32 Strep). With regard to bacterial counts, the results were expressed in MCF, equivalent to 1.5 × 108 cells/ml.