Background: Gastric cancer is the fifth most common cancer and the 3rd most common cause of cancer related mortality worldwide. Gastric carcinoma contributes to 10% of cancer deaths worldwide with a case fatality ratio of 70%. Despite recent advances in cancer treatment, the overall 5-year survival for patients with newly diagnosed gastric cancer is 28%. Objective: To study the HER-2/neu expression in gastric carcinoma patients in a tertiary care hospital. Materials and Methods: The present study is a retrospective and prospective study done to assess HER2/neu protein over expression in gastric carcinomas. Excision specimens of patients with gastric carcinoma who underwent surgery were obtained from cancer hospital and research institute were included in the time bound study.