Background: Menstrual hygiene management is an important issue for adolescent girls. Lack of education and communication regarding reproductive system further adds to the problem. Good menstrual hygienic practices such as the use of sanitary pads and adequate washing of genital area are essential during menstruation. Aims and objectives: To assess the knowledge, attitude and practice of menstrual hygiene among adolescent girls in Sothern Rajasthan India. Materials and Method: A cross sectional study was conducted among 185 school going adolescent girls of southern Rajasthan. A pre formed pre-tested questionnaire was used. Data were analyzed statistically by simple proportions. Result: Most of the girls (84.3%) attaining menarche in the age group of 12-13 years. 74.6% girls residing at rural area, 56.2% belonged to joint family and 58.4% was lower socioeconomic class. Knowledge of menstruation before menarche was found in 39.5% of girls. 84.3% menstrual habits were inculcated by their mothers.76.7% girls were using sanitary napkins as absorbent material during their menstrual cycle. 82.7% were doing vaginal wash daily. 64.6% of girls were having good menstrual practice. Conclusion: The knowledge about menstrual cycle is found to be very poor. The practices related to menstrual hygiene were satisfactory but need improvement.