In this study, 60 blood samples were collected from pregnant and aborted women infected with Toxoplasmosis, while 30 blood samples were collected from non-infected women. 5 ml of venous blood was collected and placed in SST (Serum Separating Tube) tubes for 10-15 minutes to separate the serum before centrifuged for 10 minutes at 3500 rpm. FSH and LH concentrations, as well as IL-12 and IFN-γ kinetics, were measured using ELISA. The study's findings revealed a rise in the concentrations of IL-12 and IFN-γ, which were (10.17±0.20 pg/ml) and (8.67±0.18 pg/ml) in the affected women, respectively. It was also discovered that the concentration of IL-12 did not change between the two age groups (20-30) and (40-31) for female patients, where it reached (10.15±0.25) and (10.32±0.33) pg/ml, respectively, whereas the high concentration of IFN-γ was recorded within the age group (40-31) for female patients, which amounted to (9.61 ±0.51pg/ml) compared to its concentration in the age group (20-30) for female patients, whichamounted to (8.40±0.14 pg/ml). The infected women likewise had high LH concentrations and low FSH concentrations, which were (14.85± 0.94)) and (5.20±0.607) mlU/ml, respectively. There was no difference in the concentration of LH hormone for female patients in the two age groups (20-30) and (40-31), as it was (15.08± 1.28) (14.21 0.28) mlU/ml respectively., and no difference was shown in the concentration of FSH among the female patients in the age groups (20-30) and (40-31), its concentration was (5.58 ± 0.90) and (4.11 ± 0.13) mlU/ml respectively.