Background:Thyroid hormones influence renal development, renal hemodynamic, glomerular filtration rate (GFR), electrolytes, and water homeostasis. The location of the present study is situated at dDepartment of Biochemistry, Maheswara Medical College, Hyderabad with moderate prevalence of thyroid dysfunctions, especially hypothyroidism. The objective of this observational cross-sectional study is to substantiate the effects of thyroid hormonal status on kidney by estimating serum creatinine, serum urea, albumin-to-creatinine ratio (ACR), and estimated GFR (eGFR) among primary hypothyroid patients with age- and sex-matched control group. The collected blood and urine samples from the study population have been estimated for the study parameters. Both Chronic Kidney Disease Epidemiology (CKD- EPI) equation and four- variable Modification of Diet in Renal Disease (MDRD) Study equation were used to calculate eGFR. In the present study the mean values of serum creatinine, urea, and ACR are significantly increased among untreated patients with hypothyroidism, with the decrease in the eGFR, in comparison to healthy control group (p < 0.001). The results of eGFR and ACR are signifi cantly correlated with thyroidstimulating hormone (TSH) values. Statistically significant alteration in renal function parameters is associated with untreated primary hypothyroidism. Moreover, with the initiation of the treatment for the same can cause reversal of the altered status of renal function.