Background: Diabetes mellitus is a major medical problem throughout the world. Diabetes causes an array of long-term systemic complications that have considerable impact on the patient as well as society, as the disease typically affects individuals in their most productive years. Objective: To correlate Serum Prolactin levels in patients with Diabetic retinopathy. METHODS: A total of 189 patients of diabetic retinopathy were enrolled in the study. Details of their medical and personal history, viz. age at diagnosis of diabetes, duration of the diabetes, nature and duration of treatment received, glycaemic control and compliance to the treatment were obtained. The diabetic retinopathy status was classified according to the ETDRS classification system. RESULTS: A mean HbA1C levels for each grade of DR: in Mild NPDR the HbA1c was found to be 8.33%, Moderate NPDR was found to be 8.88%, severe NPDR the HbA1c was 9.92% and for PDR the HbA1c was 11.01%. A mean Serum Prolactin levels for each grade of DR: in Mild NPDR the serum prolactin levels was found to be 8.76ng/dl, Moderate NPDR 8.57ng/dl, for Severe NPDR it was 7.28ng/dl, and it was 5.94ng/dl for PDR. A mean duration