Abstract Our research provides an update vision about the recent development in nanodrug by using eco-friendly and cost-effective silver nanoparticles from A. hypogaea root nodules for the first time. We also characterized these silver nanoparticles from various spectroscopic and microscopic methods. Their stability makes the present method a viable alternative to chemical and physical methods. Because of the least specificity of the reaction parameters, this process can be explored for large-scale synthesis of silver nanoparticles. Antimicrobial activity of silver nanoparticles room sprayer showed promising antimicrobial activity against S. aureus, E.coli and A. niger in the 100 ppm concentration and in future this kind of eco-friendly and cost effective silver nanoparticles room sprayer is needed to control many dread full diseases in the present scenario instead of using commercially available room sprayers. The investigation features a productive method to acquire bionanoaerosol that can be used against pathogenic microorganisms, thus contributing to solve this globally serious concern, especially given their being a limited choice of nanoantibiotic treatment.