Background: The present study was conducted for evaluating and comparing two different root canal irrigating solutions during root canal therapy.Materials & methods:Assessment of 20 freshly extracted mandibular first premolar were obtained and were broadly divided into two study groups with 20 specimens in each group; Group 1- Root canals irrigated using 2.5% NaOCl for 1 min only, and Group 2- Root canals irrigated with 2% CHX gluconate for 1 min followed by 3 ml of 2.5% NaOCl solution. The cleaning ability of irrigating solutions was evaluated using the smear layer score system. All the results were recorded in Microsoft excel sheet and were analyzed by SPSS software.Results: Mean smear layer score among specimens of group A and group B was 4.5 and 4.2 respectively. Non-significant results were obtained while comparing the mean smear layer score. Conclusion:From the above results, the authors concluded that both the irrigating solutions are equally effective in removing smear layer.