Introduction: It is well known that fungi can play a major role in the aetiopathogenesis of chronic rhinosinusitis (CRS) with or without nasal polyposis (NP). Objectives of the Study: Our study was aimed at looking for the presence of fungal organisms in CRS and to identify the fungal species.We also compared the efficacy of nasal wash specimen as compared to mucosa from ethmoidal sinus for detecting fungi in the nose Materials and Methods: The study design was cross sectional. A total of 94 patients of CRS with or without nasal polyposis comprised the study group. Fungal culture of nasal washings, and fungal culture and histopathology of the ethmoid mucosa specimen were carried out. Results: Nasal washspecimens of 21 patients (22%) were positive for fungus and the most common organism isolated was Aspergillus species. Specimen culture was positive in 21 cases (22%),the most common organism was again Aspergillusspecies. The histopathology was positive for fungus in 6 cases (6%) most common isolate being Mucorales. Out of 38 cases of nasal polyps, 22 patients were positive for fungal elements; it was significant by spearman’s rho coefficient p value 0.007. Conclusion: Each form of fungal rhinosinusitis has a characteristic presentation and clinical course, with the immune status of the host playing a critical role. Accurate diagnosis and targeted treatment strategies are essential to achieve optimal outcome.