Background: Hemoglobinopathies are one of the most common inherited blood illnesses in India and are one of the country's most serious public health issues. In India, it is estimated that there are approximately 45 million carriers and 15,000 babies born with hemoglobinopathies per year. The carrier rate of hemoglobinopathy in various Indian demographic groups ranges from 3 to 17 percent. Automated cation-exchange High Performance Liquid Chromatography (HPLC) has surfaced as an outstanding screening tool for identifying this abnormal hemoglobin or thalassemic conditions. So, the purpose of this study was to identify common haemoglobin disorders, in anaemia patients using HPLC.