Introduction: Osteomyelitis is pathologically defined as inflammation of bone and bone marrow due to infection with a microbial pathogen. Septic arthritis is pyogenic infection of the joint either due to direct extension from local tissue infection or more commonly as a result of bacteremia. Microbiological diagnosis in these cases can be made by culture on enriched media and PCR. As PCR detects bacterial DNA, it does not rely on the presence of viable bacteria in the sample for identification. The rapidity of PCR along with its higher sensitivity and specificity enables faster and more accurate diagnosis and treatment Objectives: To identify the cultivable pathogens by aerobic cultureandTo detect cultivable and non-cultivable organisms causing intraarticular and bone infections using broad range PCR Material &Methods: Synovial fluid and bone tissue biopsy were processed, Gram stain was performed. Culture of synovial fluid was done using BacTec system and samples were subcultured on chocolate agar, blood agar. The growth was identified by standard tests and antibiotic susceptibility pattern was assessed by modified Kirby-Bauer method and Vitek 2 compact system .All samples were started at -20˚C for PCR. Results: Of the 50 samples, 15 samples (30%) were both culture and PCR positive .3 samples were positive by PCR , but yielded no growth in the culture . This fact could be due to antibiotic effect or other non cultivable or fastidious organisms .2 samples of synovial fluid with the growth of Escherichia coli and Staphylococcus aureus in culture were negative by PCR. This could be attributed to inhibition in the PCR reaction. According to our study, the most commonly associated organism, S. aureus, showed MRSA and clindamycin resistance ( 37% and 38% respectively). ESBL production was seen in 38% of E. coli and Proteus mirabilis. Conclusion: In this study, 17 samples were culture positive, with the most common organism being Staphylococcus aureus (41%) followed by Enterobacter cloacae (18%) ,Enterococcus fecalis (12%), Pseudomonas aeruginosa (12%) and Escherichia coli (12%). Out of the 33 culture negative samples, 3 samples were PCR positive. 2 out of the 17 culture positive samples were negative on PCR. S. aureus, showed MRSA and clindamycin resistance ( 37% and 38% respectively). ESBL production was seen in 38% of E. coli and Proteus mirabilis. High rates of culture negativity may be due to empirical antibiotics given prior to sample collection. But since PCR detects DNA of the micro organism, it doesn’t differentiate between dead and living cells hence it usually picks up organisms that are missed on culture