Background:NFGNB are innately resistant to many antibiotics and have been documented to produce extended spectrum β-lactamases and metallo-β-lactamases. MDR is common and increasing among Non-fermenters. There are few studies from India that provide identification and antimicrobial susceptibility pattern of NFGNB. Therefore, we conducted this study to isolate and identify NFGNB, determine the antimicrobial susceptibility profile of isolates and estimate prevalence of multidrug resistance in NFGNB from urinary samples.