Online ISSN: 2515-8260

Quantitation of HIV1 RNA viral load and its correlation with CD4 cell count in Hepatitis C virus and human immunodeficiency virus co-infected patients attending RIMS hospital Imphal

Main Article Content

Dr. Sougrakpam ratna, Dr. Ngangom lilavati, Dr. H. Rebachandra singh , Dr. Khumanthem Shailendra Singh , Dr. Arunoday Kumar.

Abstract

Background: HCV-HIV co-infection leads to more rapid progression to cirrhosis, end stage liver disease and hepatocellular carcinoma but some literature opined that overall survival of HIV positive persons is not affected by the presence of HCV.This study was conducted with the objective to quantify HIV1 RNA viral load in HIV-AIDS coinfected individuals & its correlation with CD4 cell count. Methodology: Hospital based Cross sectional study was conducted among HIV-HCV coinfected individuals from Nov 2015 to Oct 2017.HIV1 RNA viral load was measured by the Cobas TaqMan HIV1 Test for manual specimen preparation and Cobas TaqMan 48 Analyzer (Roche Diagnostics, USA) for automated amplification and detection. CD4 cell count was measured by Fluorescent Activated Cell Sorter count system (Becton Dickinson’s immunocytometry system, San Jose, CA,USA). The correlation between HIV viral load and CD4 cell count was determined by Pearson correlation test (rp) and probability value of< 0.05 was considered statistically significant. Results: Among 47 HIV-HCV co-infected individuals analysed, maximum number i.e., 45(95.74%) have HIV viral load <10,000 copies/ml while only 2(4.26%) have ≥10,000 copies/ml. There was a positive good correlation (rp=0.611) between HIV viral load and CD4 cell count, but it was not statistically significant. Conclusions: Majority of these individuals having low HIVviral load have low CD4 cell count, some as low as 25 cells/u1. All the subjects were on ART but none on antiviral therapy for HCV. So, in these circumstances, even though they are taking ART, they are still prone to opportunistic infections

Article Details