Background:Oesophageal cancer is the eighth most common cancer and sixth in cancer related mortality, worldwide. The current study was carried out to see the role of three markers (P53, HER2, EGFR) in various oesophageal lesions, their association in cancer progression and their role as tumour markers. Materials and Methods: Study was conducted on 100 cases of oesophageal lesions consisting of both neoplastic and non-neoplastic spectrum. Immunohistochemistry was performed on all the specimens for three markers as per the set criteria and expression of these markers was studied and scoring was done accordingly. Results: Present study was conducted on 100 cases with oesophageal lesions both neoplastic and non-neoplastic. Parameters like age, gender, family history, alcohol intake, cigarette smoking and location of lesion in oesophagus were also studied. Expression of P53, Her2, EGFR were studied using IHC.