Background : This study aims to determine the effect of a combination regimen of metformin and without metforminon the conversion of sputum smear in new cases of pulmonary TB patients with type 2 DM. Materials andMethods: This cross-sectional study was conducted at Wahidin Sudirohusodo Hospital, Makassar Center for Lung Health, Kasi-Kasi, and Sudiang Community Health Center from August 2019. This study involved 83 new cases of pulmonary TB subjects with T2DM. New cases of pulmonary TB patients were obtained by examining AFB sputum in DM patients who had symptoms of pulmonary TB and had no history of suffering or receiving previous anti-TB drug therapy.Pulmonary TB patients receive category 1 anti-TB drugs andDM subjects were divided into 2 groups is metformin and non-metformin. Then, smear sputum is examined in the 2-month intensive phase of antiTB drug therapy to assess the conversion of AFB sputum. Results: From a total of 83 new cases of pulmonary TB and T2DM, 44 subjects received a combination regimen of metformin and 39 subjects non-metformin. In this study, there were 42 (95.5%) subjects who conversion sputum smear in the metformin group more higher than in the non-metformin group but statistically it was not significant (p = 0.282). Conclusion: There was no significant relationship between the metformin and nonmetformin groups on the conversion of AFB sputum smear in new cases of pulmonary TB patients and T2DM.