Indigofera Aspalathoides has been an important ingredient to prepare oils used for the skin diseases and studies with the stem extract demonstrates that it has antitumor, antiviral and antibacterial effect. In our study chemopreventive effect of ethanolic and chloroform extract of Indigofera aspalathoides(EIA) and (CIA)was tested on DMBA induced buccal pouch carcinoma in hamster . The biochemical analysis of Phase I xenobiotic enzymes and histopathological examination of oral tissues were done using gold standard methods.The activity of Phase 1 enzymes were significantly elevated in he buccal pouch of DMBA painted animals when compared to control animals. The expression of CYP1B1was significantly elevated in the buccal pouch of DMBA painted animals when compared to control animals. Also the expression of AHR, ARNT and CYP1B1 in buccal pouch showed a significant elevation in the level of these protein in the DMBA alone treated group when compared to control animals. This study shows that there is a lack induction of cytochrome P4501A (CYP1A) and other aryl hydrocarbon receptor (AHR)-dependent responses after exposure to AHR agonists, suggesting an overall down-regulation of the AHR signaling pathway.