Background: Sepsis and septic shock cases in the Neonatal intensive care unit (NICU) and Pediatric intensive care unit (PICU) remain one of the most significant causes of morbidity and mortality in pediatric patients. Therefore, studying and comparing the clinical features and outcomes of pediatric patients with sepsis in ICUs are important, especially in developing countries. Methods: From 1st July 2021 to 31st December 2021, we have collected data from both of our pediatric ICU and neonatal ICU of Shyam Shah Medical College using a preformed proforma. Complete blood count, C - reactive protein and culture sensitivity reports were used to diagnose or screen sepsis. We compared clinical features, laboratory data, microbiologic results, and final outcome for patients with sepsis in both NICU and PICU.