Polycystic ovarian syndrome (PCOS) is a common endocrinopathy in women of reproductive age group and an incompletely understood enigmatic disorder of heterogenous nature. It starts appearing at 18 to 45 years of age and it may take years for its clinical presentation to appear. Anti-Mullerian hormone (AMH) has a glycoprotein dimer structure and is a member of the transforming growth factor-β (TGF-β) family. AMH is produced by the granulosa cells surrounding preantral and antral follicles and has an important role in the development and maturation of follicles. Ovulatory women with the polycystic morphology can have increased LH/ FSH ratios; however, a single blood sample can fail to detect an increased ratio. With this background, the present study was undertaken to identify and assess the biochemical test in the form of antimullerian hormone, LH/FSH ratio, serum prolactin levels in patients with clinical features of polycystic ovarian syndrome.