Online ISSN: 2515-8260

Author : Hadi, Najah R

Impact of Gene Polymorphism of Lipoprotein Lipase on Atorvastatin Treatment Outcome in Ischemic Stroke Patient in Najaf Governorate

Sarah Ali Abbas; Ahmed M. AlMudhafar; Thekra A. Al-Kashwan; Hayder K Hasson; Adhwaa Hameed Jaber; Najah R Hadi

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 225-228

Background: Stroke was commonly identified as a neurologically impairment that occur in central nervous system on vascular basis as Acute focal injury. Statin (HMG-CoA reductase Inhibitor), clinical trials in many large scaled shown that in primary and secondary CVD the used of statin was declined rates of (CV). Human Lipoprotein Lipase (LPL) gene coding was place in short arm of chromosome in the p22 region of the same Chromosome and has nine introns and ten axons. Aim: The aim of the study is to find out effect of LPL gene polymorphism of response to atorvastatin treatment in patients with ischemic stroke. Patients and methods: Samples was Picking up from patients’ admission to at Middle Euphrates Center for Neurological Sciences in the main hospital for measurement of the lipid profile, molecular analysis study of genotyping and measurement of human lipoprotein lipase by ELISA technique. Result: There is good response to treatment and the response consider statistically significant, the best group response to treatment was homozygous mutation then Heterozygous mutation and the less group response was Homozygous normal, wild. Conclusion: The effect of drug on serum biomarker not affected by age except HDL (best result in age<60yr), and not affected by gender and weight state, while in consider to smoking and Hypertension TG (best response in non-smoker, hypertensive) and VLDL (best response in smoker, hypertensive) affected and in diabetic state the Cholesterol and TG affected on response (the best result in non-diabetic). Heterozygous mutation most distributed then homozygous normal and the less distributed was homozygous mutation.

Synthesis and Evaluation of The Thrombolytic Activity of Novel Condensed Pyrimidine Sulfonamide Derivatives

Mazin M. Mohsin; Mahmood J. Jawad; Saif M. Hassan; Samir M. Awad; Yassmine Ali Hussain; Najah R Hadi

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 220-224

Pyrimidine-5-sulfonamides are new organic compounds of wide spectrum activity. A new trend is to incorporate a salicylate moiety in an attempt to enhance a thrombolytic activity. We started this strategy using 2-thiouracil 1 as a blocking unit by its ability to be chlorosulfonated at 5th position by refluxing with chlorosulfonic acid giving sulfonyl derivative 2 which was condensed with three common aromatic amines namingmethyl-4-aminosalicylate, methyl-3-aminobenzoate and methyl-4-aminobenzoate respectively yielding sulfonamide derivatives 3a-c. Moreover, 3a-c derivatives were reacted with chloroacetic acid-producing thiazolopyrimidines 4a-c,which in turn were reacted with p-nitrobenzaldehyde giving arylidine derivatives 5a-c. Also compounds 3a-c were cyclocondensed with anthranilic acid yielding pyrimidoquinazoline derivatives 6a-c. They also were condensed with ethanolamine forming imidazopyrmidine derivatives 7a-c. All the newly synthesized compounds were subjected for thrombolytic evaluation and some of them showed promising activity especially those bearing methyl salicylate moiety as compared to ticlopidine and clopidogrel. We performed different modifications in the thienopyridine chemical structure of ticlopidine and clopidogrel to conclude that the presence of second nitrogen atom in the pyridine ring (yielding pyrimidine moiety) and the presence of the other cycle instead of thienyl ring would lead to enhanced the thrombolytic effect which was maximized by incorporation of a methyl salicylate moiety. We concluded that 4a, 6a, 8a, 7a, 3a, compounds have more potent than both clopidogrel and ticlopidine respectively. They certainly deserve further studying in the future.