European Journal of Molecular & Clinical Medicine

The definition of death lost its significance in favor of brain death. Brain death is a permanent cessation of all functions of the brain in which though individual organs may function but lack of integrating function of the brain, lack of respiratory drive, consciousness, and cognition confirms to the definition that death is an irreversible cessation of functioning of the organism as a whole. In spite of medical and legal acceptance globally, the concept of brain death and brain-stem death is still unclear to many. Brain death is not promptly declared due to lack of awareness and doubts about the legal procedure of certification. Many brain-dead patients are kept on life supporting systems needlessly. an attempt has been made to highlight the history and concept of brain death and brain-stem death; the anatomical and physiological basis of brain-stem death, and criteria to diagnose brain-stem death in India.

To evaluate the application of mitomycin-C on the density ofcorneal endothelialiumafter photorefractive keratectomy (PRK) in myopia patients. We use case control study to evaluate 92 eyes of 46 patients (23 cases and 23 controls) with mean age of 27.6 years for cases group and 27.4 for control group (range: 20 - 40). All patients were myopic ranged from -3.00 to -6D underwent photorefractive keratectomy (PRK). The first group (cases) was treated with single usage ofmitomycin-c for 30 seconds and second group (control) group was treated without mitomycin-c. The assessment was done by specular microscope before the procedure and after 3 months postoperatively for each eye to evaluate the density in corneal endothelialium (ECD) in both each group. Forty-six eyes of 23 cases and forty-six eyes of 23 control patients with myopia were treated with photorefractive keratectomy. After three months of surgery the mean ECD was decreased significantly.Theuse ofmitomycin-c for 30 seconds in myopic patients treated by PRK would affectcorneal endothelial cell density after three months postoperatively.