Online ISSN: 2515-8260

Author : AMALDAS, JULIUS


ERYTHROCYTE TRANSMEMBRANE FLUX AND MEMBRANE TRANSPORT ABNORMALITY IN PATHOGENESIS OF HYPERTENSION LEADING TO NEPHROLITHIASIS

RENUGADEVI KARTHIKEYAN; JULIUS AMALDAS; PRAKASH DHARMALINGAM

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 1698-1705

The epidemiological relationship between nephrolithiasis and hypertension is well-known. Patients with hypertension are at increased risk for nephrolithiasis and those with nephrolithiasis are at risk for hypertension. An anomaly in RBC Oxalate transport and reduced activities of adenosine triphosphatases has been reported in patients with hypertension when compare to control subjects. This study presents an abnormal increase in transmembrane flux of oxalate in RBC of hypertensive subjects and it might be due to membrane degradation caused by oxalate-induced free radicals depleting erythrocyte thiol contents and impaired adenosine triphosphatases activity resulting in tissue injury and defective membrane transport. Thus relative risk of hypertension was significantly associated with increased oxalate flux rate and impaired adenosine triphosphatases activity in stone formers. This association is important when treating patients with nephrolithiasis since those with hypertension may require unique dietary and medical therapy.

Artemisia annua L. silver nanoparticles exhibit anticancer activity by ROS mediated apoptosis in MDA-MB-231 cells.

RENUGADEVI KARTHIKEYAN; JULIUS AMALDAS

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 1686-1697

Aim of this study was to investigate the growth inhibitory effect of Artemesis annua L. silver nanoparticles (A.annua AgNPS) on human triple negative breast cancer tumor cell line MDA-MB-231. Cells were cultured and treated with A.annua water extract and A.annua AgNPS to determine the half inhibitory concentration (IC50). Oxidative stress was determined using lipid peroxidation (LPO), ROS generation and by determining the levels of antioxidants, superoxide dismutase (SOD) and reduced glutathione (GSH). Further, the mechanism of apoptotic induction was assessed by DNA fragmentation analyses. We observed that A.annua AgNPS significantly caused growth inhibition by increasing levels of LPO and ROS, in a dose dependent manner with reduced level of endogenous antioxidant enzymes. Increased shear in genomic DNA from MDA MB231 cells treated with A.annua AgNPS shows apoptosis to be the cause of cell cell death. We conclude that A.annua AgNPS can be useed as a therapeutic agent against breast cancer.