Online ISSN: 2515-8260

Author : A.D., Dusanov

Distribution Of Alleles And Genotypes Of Noesg3 Genes Polymorphism Among Individuals With Bronchial Asthma In The Uzbek Family

Okboev T.A.; Dusanov A.D.; Yuldasheva D.A.; Zakiriayeva P.O.

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 3430-3441

Examined in the family of patients suffering from bronchial asthma in 49 probanda and 346 relatives in I - IV generations. Relatives of the proband in the family are divided into 3 groups: in the first group, relatives suffering from BA (82/346 - 23.70%); in the second group, relatives with allergic diseases (allergic rhinitis, atopic dermatitis, urticaria) (81/346 - 23.41%) and the third group, healthy in the family (183/346 - 52.89%). Family members from 4 to 78 years old comprised 395 people, of which 186 (47.09%) were men and 209 (52.91%) women. The average age was 33.55 years. The polymorphic variant of NOESG3 gene was analyzed by polymerase chain reaction of DNA synthesis (PCHR) on amplifier and RFLP-analysis with subsequent electrophoresis in polyacrylamide gel. These studies were carried out in the "Center of Medical Genetics" of the Institute of Biochemistry of the AcBAemy of Sciences of Uzbekistan. Carriers of NOESG3 gene consisting of N/N alleles of homozygotes were detected more frequently in the Uzbek population among patients with family BA, which testifies to hereditary disease in patients with this genotype. This genotype is more common in controlling patients and women, and a milder degree of disease was diagnosed. Also in patients with this genotype was diagnosed allergic BA, which recorded an increase in the number of total IgE and IL-6, a decrease in IFN-γ. It was noted that among patients with family BA in the Uzbek population heterozygous carriers consisting of N/del alleles of NOESG3 gene were significantly rarer, and more often observed in healthy men. In the family it was shown that in patients with this genotype of controlled asthma the total amount of IgE increases, and in uncontrolled patients a reliable increase of IFN-γ was observed, which is a diagnostic marker. It was noted that in the Uzbek population carriers of homozygous genotype del / del NOESG3 gene are more often found in patients with non-allergic type of family BA than in uncontrolled and severe forms of disease.