Online ISSN: 2515-8260

Author : ALOK KUMAR SRIVASTAV, POONAM JAISWAL,


AN ANALYTICAL STUDY ON PRODUCTION AND CHARACTERISATION OF EXTRACELLULAR ANTIMICROBIAL PEPTIDES OF LACTOBACILLUS

POONAM JAISWAL, ALOK KUMAR SRIVASTAV

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 3873-3887

Probiotics provide several health benefits that transcend basic nutritional requirements of our daily meal. The focus of this study is to evaluate the antimicrobial capability of probiotic bacteria isolated from commercially available yoghurts in Indore city. Three cans of different branded yoghurts were purchased from local supermarkets and prepared using standard procedures. The samples were transferred into De Man, Rogosa and Sharpe (MRS) broth and incubated anaerobically for 24 hrs. The isolates were identified using biochemical tests. The antibacterial activity was evaluated using agar diffusion assay. The effects of pH and temperature on the growth of isolated Lactobacillus spp. as well as their ability to tolerate bile salt were assayed. All isolated lactobacillus spp. has inhibitory effect on E. coli, S. aureus and P. aeruginosa. Bile salts incorporated in the bacteria cultures were effective on bacterial viability. The present study also focused on the antimicrobial activity of cell free culture filtrate and extracellular protein concentrate of L.actobacillus strain against food-spoilage organisms and human pathogens. Though  both  bacteria  and  fungi  that  have  beneficial  health effects are categorized as probiotics, Lactobacillus spp. have been well characterized as prominent members of the probiotic group. The probiotic bacteria isolated in commercially available yoghurts have health beneficial effects, which include antibacterial activities.
Keywords: Mycobacterium tuberculosis, Tuberculosis, Aspartate pathway, Metabolomics, Persistent infection, Aspartate kinase, Feedback inhibition, Combination therapy, Multi-drug resistant tuberculosis (MDR-TB)