Author : Agarwal, Dr. Amit Kumar Khajuria, Dr. Mohit Thalquotra, Dr. Parul Singh, Dr. Gaurav
ASSESSMENT OF OXIDATIVE STRESS GENERATED BY ORTHODONTIC ARCHWIRES- AN IN VITRO STUDY
European Journal of Molecular & Clinical Medicine,
2022, Volume 9, Issue 7, Pages 2092-2096
Introduction: Numerous metals go through redox cycling, which results in the production of free radicals and oxidative stress. This study looked into the in-vitro oxidative stress placed on orthodontic archwires comprised of different alloys.
Methods: The quantity of the oxidative stress marker 8-hydroxy-20-deoxyguanosine in DNA was measured after exposing mouse fibroblast cells L929 to 6 different types of archwires. Cell vitality and number were determined using trypan blue dye.
Results: Coated nickel-titanium, copper-nickel-titanium, and cobalt-chromium produced oxidative stress levels that were lower than those of standard nickel-titanium archwires (P <0.05), but higher than those of titanium-molybdenum and the negative and absolute controls (P <0.05). Standard nickel-titanium archwires generated the highest oxidative stress, significantly higher than all other wires and the controls (P The least tension was produced by stainless steel and titanium-molybdenum. The lowest viability was caused by nickel-titanium, which was lower than the negative and absolute controls, all other wires, and titanium-molybdenum (P< 0.05). The material with the highest viability was stainless steel. The largest suppression of cell growth was caused by nickel-titanium, which was higher than all other samples (P< 0.05) excluding the positive control and cobalt-chromium. The positive control, cobalt-chromium, nickel-titanium, and titanium-molybdenum showed the lowest inhibition (P< 0.05), followed by stainless steel.
Conclusions: Every orthodontic archwire causes oxidative stress in a test tube. The maximum biocompatibility is found in stainless steel archwires, and the lowest is found in nickel-titanium