Online ISSN: 2515-8260

Keywords : Pseudomonas


A RESEARCH INTO THE CHANGING PATTERNS OF ANTIMICROBIAL RESISTANCE

Ashok Prasad, Amit Anand, Hanspriya Bhagat, Atanu Ray

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 7, Pages 2505-2509

Introduction: A key diagnostic material for aerobic growth and susceptibility testing in the microbiology laboratory is pus. It is crucial to periodically conduct investigations to ascertain the evaluate the safety of microorganisms since the rate of emergence of resistance in bacterial isolates considerably outpaces the frequency of subsequent drug discovery and development.
Aim: This investigation was done to assess the evolving pattern of antibiotic resistance in different pus specimens.
Materials and Methods: Pus specimen was obtainedfrom several Department of Microbiology, MGM Medical college n Lion’s Seva Kendra, Kishanganjover the course of six months using an aseptic approach, and they were processed right away in the lab using conventional antimicrobial techniques. Motility tests, Gram staining, and biochemical processes were used to microbes. All specimens underwent Kirby Bauer's disc diffusion method antibiotics susceptibility testing on Muller Hinton agar, with results interpreted in accordance with CLSI recommendations.
Results: 120 pus specimens were analysed, and the results revealed that surgeries departments (32.42%) contributed the most to the 93.26% culture positive. One of most prevalent organism was Pseudomonas, followed by Staphylococcus aureus (22.51%). Gram negative bacilli were responsive to Imipenem (87.09%), Piperacillin (61.28%), and Gentamicin (48.38%) while Gram positive cocci were susceptible to Linezolid (94.86%), Vancomycin (92.30%), and Imipenem (92.30%).
Conclusions: As per regions and growing multi-resistant bacteria, the shifting patterns of antibiotic sensitivity in isolated strains from pus might be a useful tool for doctors to begin empiric therapy of patients as soon as possible

Clinico-bacteriological and antibiotic drug resistance profile of Chronic Otitis Media: Mucosal disease, at a tertiary care hospital in rural Haryana: A retrospective observational study

Garima Yadav, Mayank Yadav, Pooja Singla, Nisha Sharma, Sulabha M Naik

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 6, Pages 648-655

Background: Objectives: To determine the microbiological profile and antimicrobial susceptibility pattern of bacterial isolates from aural swabs in patients with CSOM. Materials and Methods: A total of 142 patients of CSOM with unilateral or bilateral ear discharge who attended the outpatient department from March 2020 to November 2020 were included. Results: Among 142 patients included in the study, most common age group was 11-20 years. CSOM was found to be more common in females (56.33%) than in males (43.66%). The most common bacterial isolate was Pseudomonas aeruginosa (45.8%) followed by Staphylococcus aureus (30.5%), Proteus mirabilis (13.8%), Acinetobacter baumannii (2.7%), Klebsiella pneumonia, Micrococcus, E. Coli, Citrobacter and Providentia species. Pseudomonas aeruginosa was found to have maximum  sensitivity to Polymyxin B (87.8%) and least sensitive to amoxicillin (15.1%). Among Staphylococcus aureus (87% MRSA and 13% MSSA) maximum isolates were sensitive to piperacillin+tazobactam (90.9%) and least sensitive to amoxicillin (13.6%).

Change in findings of culture and sensitivity data if any in chronic otitis media during COVID pandemic

Raghvendra Singh Gaur, Shammi Kumar Jain, Sanjay Agrawal, Dr. Swati Chandel

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 2, Pages 1067-1073

Background: Chronic otitis media is an important cause of morbidity affecting all ages leading to variety of complications which has major impact on the patients and more so in Covid pandemic. Study to see the change in findings of culture and sensitivity data in chronic Otitis media during Covid pandemic and hence modify the treatment plan.
Aims: To study the bacteriology associated with the chronic otitis media and study any change in findings of bacteriology of chronic otitis media during COVID pandemic.
Subjects and methods: The retrospective study was done. The culture and sensitivity data during Covid pandemic was collected from Jan 2020 to Jan 2021 from ENT centre and their analysis and interpretation done according to age, types of culture and their sensitivity.
Result: In the total 100 cases of culture and sensitivity results during pandemic, pseudomonas aeruginosa was the most common organism isolated and showed highest sensitivity towards piperacillin/tazobactam and polymyxin B. Staphylococcus aureus was second most common which showed highest sensitivity towards linezolid and teicoplanin. Both showed lowest sensitivity towards ciprofloxacin. There was no change found in the bacteriology culture during the Covid pandemic which was compared from available literature.
Conclusion: the change in the findings of culture and study of chronic otitis media based on the study done shows almost no difference with the pre-pandemic times. But high antibiotic resistance implicates the careful use of antibiotics in treatment and avoiding empirical method of treatment.

VIRULENCE MARKERS ANDMOTILITY PATTERNS EXHIBITED BY PSEUDOMONAS SPP. ISOLATED FROM HIV AND NON-HIV POPULATION WITH LRTI.

Anitha C; Senthamarai S; Sivasankari S

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 1002-1014
DOI: 10.31838/ejmcm.07.09.101

Detection of biofilm, slime and motility patterns in Pseudomonas spp. can be valuable in empathetic the virulence of the creature. Here studies on the phenotypic methods of biofilm production, slime production and the motility patterns exhibited in 69 clinical isolates of Pseudomonas spp. and 2 environmental isolates were performed. Nineteen strains produced biofilm in which 6 were from HIV and 13 were from Non-HIV patients. All the 19 isolates positive for biofilm production were taken for detection of biofilm genes by PCR. The gene coding for alkaline protease aprA gene was detected in 13 (68.42%) isolates. The gene for phenazine biosynthesis phzA1 gene was detected in 14 isolates. The house keeping gene acpP was found to be positive for 18 (94.7%) isolates. 48 isolates were found positive for slime production. Pellicle formation was positive in 39 isolates. Swarming motility was positive in 42 isolates 29 were from HIV and 11 were from Non-HIV. Swimming motility was exhibited in 31 isolates. Twitching motility was exhibited in 35 isolates. The 2 environmental isolates were found to be positive only for slime production, swarming, and swimming motility. Among the clinical isolates, HIV isolates showed high production of slime, pellicle and positivity for the motility patterns seemed to be high in HIV compared to Non-HIV isolates except for the biofilm production which is high in Non-HIV isolates.