Online ISSN: 2515-8260

Keywords : Cytology


Dr. Harsh Jakhetia, Dr. Smriti Pandey, Dr. Kamlesh Patel, Dr. Dhruvendra Pandey

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 1019-1026

Background: For the evaluation and assessment of both non-gynaecologic and gynaecologic
specimens, extensive use of LBC (Liquid-based cytology) has gained popularity in the recent
past. Better assessment morphologically is possible owing to the monolayer cell suspension.
Also, LBC allows assessment of nucleolar prominence, smaller cell size, fragmented cell
clusters, lost, reduced, or altered background material.
Aim: The present study was conducted to comparatively evaluate the utility and efficacy of
Liquid-Based Cytology over conventional smears for assessment of the breast lesion, and to
assess their use as an alternative to the conventional preparation.
Materials and Methods: The present prospective clinical study included 374 female subjects
of the FNAC (fine-needle aspiration cytology) obtained from subjects having palpable breast
lesions. The second pass was given, whereas, the first pass was used for either LBC or
conventional smear. Several criteria were used to compare representative LBC and
conventional smear. Individual scoring of each criterion was done followed by statistical
Results: The study results showed that statistically non-significant difference was seen
between LBS and conventional concerning nuclear and cytoplasmic details, presence of
monolayer,cytoarchitectural pattern, and cellularity, whereas, a statistically significant
difference was seen for background blood-debris and informative background.

Role of Tzanck Smear microscopy for detection of vesiculobullous skin lesions and active genital herpes- a pilot study in tertiary care hospital, Patna, Bihar

1Randhir Kumar, 2Kranti C Jaykar, 3Nidhi Prasad, 4Shailesh Kumar, 5Rakesh Kumar, 6Namrata Kumari .

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 3326-3331

Background: Cytology is a diagnostic technique used to evaluate the properties of individual cells. Tzanck smear is a quick, simple, sensitive cytology procedure that can be done with little patient discomfort and expense.
Objective: The purpose of this study was to determine the value of Tzanck smear in evaluating vesiculobullous illnesses and active genital herpes in relation to clinical diagnosis.
Methods: A cross-sectional study was carried out to determine the value of Tzanck smear in evaluating various vesiculobullous skin lesions in comparison to clinical diagnosis. For statistical analysis, the Fisher's exact test was applied.
Results: The majority of the 70 patients evaluated had viral infections or auto immune vesiculobullous diseases. On cytology, all patients with viral infection had multinucleated giant cells, and all patients with pemphigus had a large number of acantholytic cells as well as an inflammatory infiltrate. All of the patients with bullous pemphigoid had a significant number of eosinophils. The presence of acantholytic cells, multinucleate large cells, and eosinophils had a highly significant connection with clinical diagnosis.
Conclusion: Tzanck smear, while not a replacement for traditional histology, can aid in establishing the clinical diagnosis with ease and speed.


B. Sabarinath; Preethi Sundaraman; B. Sivapathasundharam

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 1015-1019
DOI: 10.31838/ejmcm.07.09.102

Background: Tissues are fixed and preserved and protected from putrefaction and autolysis before routine histopathological or cytological examination. Ethanol is commonly used as a cytological fixative due to its better preservation of nuclear details. Honey, a natural product which possess antibacterial; acidic; dehydrative properties which classify it as fixative than a preservative. Aim:The aim of this study is to compare ethanol with honey as a cytological fixative. Materials and Methods: Smear samples of buccal mucosa from patients submitted to the Outpatient Department of Oral Pathology and Microbiology, Meenakshi Ammal Dental College and Hospital, Chennai, were included in the study group. The study consisted of two groups. Group I that used alcohol as the fixative comprising of 30 smear samples and Group II that used 10% honey to fix 30 smear samples.A double-blind study was conducted. Statistical Analysis: Independent t-test was the statistical method applied for the study using SPSS software (Version 17). Result: The results are discussed. Conclusion: Honey can be used asan alternate fixative to alcohol in cytopathology.