Online ISSN: 2515-8260

Keywords : Eugenol

Insilico Interaction of Bioactive Compounds from Clove againstOral Candida albicans biofilm drug targets

Sindhu priya Kuppusamy; Lakshmi T; Surendar A

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 1, Pages 537-548

Aim: Insilico Interaction of Bioactive Compounds from Clove againstOral Candida albicans biofilm drug targets. Materials and methods: All the 3D models were obtained from PubMed and the final analysis was produced by two systems, mainly chemsketch and GOLD protein-ligand docking. Results : Here in this study we have tried to find the best compatible bio active compound of clove towards the target. Out of 5 Bioactive compounds from clove Eugenol acetate O4,O3 showed the highest docking score having h bond score to be 2.888(O4) , 2.653(O3). Conclusion: In most of the cases the docking the H bond value must be considered must be considered because the hydrogen bonds are stronger than van-der-walls bond and weaker than covalent bond as H bonds have the ability to create a bond or break a bond easily in this study we r trying to read the compatibility between the bioactive compounds and the target compound in this case its Candida albicans biofilm. Further the the research can be extended to wet lab work for further details.


Happy Kurnia P; Dhanang Puruhita T R; Muhammad Nazhif H; Rizq Threevisca C

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 1184-1197

Eugenol is a phenylpropanoid group compound found in cloves, nutmeg,
cinnamon, and bay leaves. Apart from being used as a cosmetic, perfume, and food
ingredient, eugenol is known to have an antioxidant, antibacterial, anti-inflammatory, and
anti-cancer profile. Eugenol has therapeutic potential by increasing reactive oxygen
species formation, decreasing anti-apoptotic protein Bcl-2, increasing the release of
cytochrome c that leads to apoptosis in cancer cells, and inhibit the epithelial to
mesenchymal transition (EMT) process that could reduce the cell ability to migrating.
We synthesized eugenol loaded chitosan nanoparticles (Nano-EU) by ionic gelation
method to overcome its shortcoming which is volatile and to increase its bioavailability.
The nanoparticles were characterized by using Dynamic Light Scattering (DLS).
Anticancer activity of Nano-EU was investigatedin cervical cancer HeLa cell line by flow
cytometry using Annexin-V/PI staining, and by measuring cleaved-caspase-3 protein
expression which is the executor of the apoptosis process by immunofluorescence.
The results of the study evidenced that Nano-EU inducing apoptosis and increasing
activated caspase-3 expression in HeLa cells. Nano-EU could also inhibit cell migration by
reducing vimentin and Snail as mesenchymal markers leading to inhibition of the EMT
process. Further research is still needed to investigate the anticancer potential of Nano-EU
in HeLa cells to in vivo and clinical studies.