European Journal of Molecular & Clinical Medicine

Introduction: The primary objective of any root end filling material is to seal the apical
region to avoid any kinds of bacterial infiltration or their by-products from the
supporting periodontal structures into the root canal system.
Materials and Methodology: For the study purpose, sixty mandibular premolars were
selected which were extracted for orthodontic reasons. The root surfaces were sought to
be cleaned and the soft tissue remnants present as debris were removed help of
Ultrasonic scaler and teeth were stored in distilled water until further use. The
materials that were intended to be studied in this research were manipulated as per the
manufacturer’s instructions and the cavities were filled respectively. The specimens
were then coated with 2 coats of nail varnish except at the 1 mm apical area & then they
were allowed to be dried.
Results: Group A: Since it is a control group, no material was placed. All
stereomicroscopic images for the control group demonstrated a relative amount of dye
penetration. Both Group B and C showed microleakage, where Giomer and Biodentine
showed microleakage but the difference was not statistically significant. Biodentine is
considered as better root-end materials since it showed lesser microleakage than
Giomer.
Conclusion: Both the root-end filling materials such as Giomer and Biodentine revealed
the presence of microleakage but the results were not observed to be statistically
significant. Comparatively Biodentine possessed lesser microleakage than Giomer and it
is considered to be better. Therefore, it can be effectively used as a retrograde filling
material with better results.

Background: An endodontic treatment aims to recover and maintain dental elements, which have pulpal or endodontic periapical pathology, thus eliminating the microorganisms to ensure an acceptable apical seal. Objectives: The three different conditions using naked vision, loupes and Dental operating Microscope were compared here to test the sealing ability of biodentine using glucose penetration method. Methods: Ninty mandibular bicuspids were selected that were further divided into three groups (n=30) for which canal were prepared using F2 ProTaper Universal (Dentsply Maillefer, Switzerland) and a retrograde cavity is prepared in each of the extracted single rooted tooth using proultra surgical tip (Dentsply, USA) 3mm short of apex . Cavity was then filled with biodentine condensed against plugger placed inside the canal. All the procedure were confirmed under unaided vision/ naked vision, under dental loupes (IDS Denmed 3.3X). and under Dental operating Microscope (Carl zeiss 12.5X) for each group. All the samples were then analyzed with a spectrophotometer at a suitable wavelength. The (ANOVA) test of comparison was analysed for multiple groups preceded by the post hoc pair-wise comparison test. Results: The filling of biodentine is observed under dental operating microscope that showed better sealing properties when compared to the unaided vision and dental loupes, thus proving statistically significant. The glucose leakage evaluated under working conditions namely unaided vision, dental loupes and Dental operating Microscope were 0.236, 0.111 and 0.055 respectively. Conclusion: The restoration done under Dental operating Microscope showed superior sealing properties when compared to the other working conditions respectively.