Online ISSN: 2515-8260

Keywords : HPLC


SIMULTANEOUS ESTIMATION OF ARTEMETHER AND LUMEFANTRINE IN COMBINATION DRUG PRODUCTS BY RP-HPLC METHOD

Meka.Srinivasa rao; Dr.K. Rambabu

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 11, Pages 8347-8367

This abstract illustrate an precise, isocratic RP-HPLC strategy have been
created by the creator for the concurrent estimation of artemether and lumefantrine in
unadulterated and advertised details by utilizing Inertsil C18 column (250×4.6 mm, 5μ)
improved mobile phase containing phosphate buffer(pH 4.5) and acetonitrile in the extent
of 40:60 %v/v and Discovery wavelength at 218nm. The retention times were 2.207min
and 3.733min for artemether and lumefantrine respectively. The linearity range was
found to be 10-30μg/ml for artemether and 20-60μg/ml for lumefantrine individually. The
developed method was Validated for specificity, system suitability, precision, linearity,
accuracy, Limit of Detection, Limit of Quantification, robustness, and Stability and the
examine results acquired for all the approval parameters by this proposed strategy were in
reasonable concurrence with ICH standards. Thus, the developed RP-HPLC method
represents another good alternative for the already existing HPLC methods especially
those using certain types of detectors which are not present in most of the laboratories

Estimation Of Phenolic Compounds And Evaluation Of Their Antioxidant Activity Of Some Parts Of The Orange Plant (Citrus Sinensis L.)

Iman Mohsin Kadhom; Nagham Saddon Ibrahim; Najim Abdullah Al-zubaidy

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 4811-4822

Orange fruits were collected from one of the gardens of Baqubah / Diyala / Iraq in January of 2020, while samples of leaves and flowers were collected in March of the same year, for the purpose of completing the research procedures. The present study aims to estimation of phenolic compounds and evaluation of their antioxidant activity of the extract of some parts of the orange plant. The results show a variation in the parts of the orange plant in their content of phenolic compounds. Moreover, the statistical analysis pointed to the presence of significant differences at the probability level of P <0.05 among the parts of the orange plant, in Total Phenolic Content (TPC) and Total Flavonoid Content (TFC). The leaves are superior in their (TPC) which record the highest in comparison with the juice, which recorded the 1-concentration of 145.71 mg( GAE) .g. Though the highest (TFC) in the mesocarp of fruit is 1-lowest (TPC) 57.18 mg( GAE) .gin the juice, which gave the 1-compared to 5.81 mg.Querecetin. g 1-mg Querecetin .g 11.14lowest (TFC).
On the other hand, the result of statistical analysis showed significant difference among the parts of orange plant at the probability of P <0.05 in antioxidant activity. The result showed that the highest antioxidant activity, detected by using the DPPH scavenging . While the lowest value of the free radicals DPPH 1-test of the flowers is 2.64 mg. mLwas obtained from exocarp. The statistical analysis 1-inhibition, which was 0.86 mg. mLresults indicated a positive correlation among the (TPC), (TFC) and the antioxidant activity of DPPH free radical test with value of 0.385 and 0.484 respectively.

Method Development And Validation Of Cetirazine Hcl And Montelukast Sodium

Vancha Harish; Sanjeev Sahu

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 5855-5874

The detection, quantification of pharmaceutical products is mainly done by a dominant technique known as HPLC. Chromatographic techniques is used to analyze the parameters and optimize the parameters which are used in the method development and its validation. The present work mainly focuses on the method development and its validation of the Montelukast sodium and Cetirizine hydrochloride. All the parameters optimized has given the précised values.