Online ISSN: 2515-8260

Keywords : Gene expression

The Effects of Purified Staphylococcal Enterotoxin A on Gene Expression of Nuclear Factor Кappa B (NF-КB) in Rabbits

Ayat Ali Mahmood; Ahmad Darweesh Jabar; Muthana Ibrahim Maleek

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 4319-4324

This study aimed to determine the effects of Staphylococcal enterotoxin A on NF-KB gene expression in rabbits. Firstly, SEA was isolate, purified and identified from one strain of Staph-ylococcus aureus collected from urine sample, and had identified by biochemical and molecu-lar methods. Then ,Staphylococcal enterotoxin A was purified by chromatography technique . All purified fractions of SEA was determined by ELISA kit. Finally, 5mg\ml of SEA was injected intravenously to rabbits in order to demonstrate its effects on NF-KB gene expression. At the end of this experiment, the gene expression of NF-KB increased to 9.1 in compression with control group with 1 of fold time (P = 0.045)

Cloning And Analysis Of The Nile Tilapia Toll-Like Receptor Type 5 Mrna Sequence

Reham R. Abouelmaatti; Mohamed Rady; Xiaokun Li; Jisheng Ma; Wael M.K. Elfeil

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 3956-3967

Toll-like receptors (TLRs), which detect infections in vertebrates, are the most thoroughly understood innate immune receptors. However, bony fish’s TLRs shows distinct features and substantial diversity, which are likely originated from the variation in evolutionary history of fish and the distinct environments that they life in. there are a limited data about the structure of the fish immune system. Our work aimed to identify and clone and sequence the Nile tilapia TLR5 for the first time as a model for freshwater fish species. The full-length sequence of Nile tilapia (Oreochromis niloticus) TLR-5 receptor has been identified, where it consisted of 2661 nucleotides. The consensus cDNA sequence showed 81% identity with the sequence from Takifugu rubripes, 77% identity with the sequence form Japanese flounder, and 75% identity with the sequence from Japanese medaka, which confirmed that the new sequence is considered probably homologous to fish TLR-5. The predicted protein structure encoded by the Nile tilapia TLR-5 mRNA composed of 887 amino acids, beginning with signal peptide as ATG, like other functional fish TLR-5 sequences. Analysis of the deduced amino acid sequence indicated that Nile tilapia TLR-5 has typical structural features and contains the main components of proteins in the TLR family. Our results reveal a complete and functional Nile tilapia TLR-5 that is orthologous to other vertebrate receptors

Increased expression of oxidative phosphorylation genes in breast cancer cells

Jolanta Rzymowska; Ludmi&;a Grzybowska-Szatkowska; Ludmi&;a Grzybowska-Szatkowska; Ludmi&;a Grzybowska-Szatkowska; Brygida Slaska; Izabella Cisek; Katarzyna Wach; Sebastian Mazur; Paulina Stachyra; Ludmi&;a Grzybowska-Szatkowska

European Journal of Molecular & Clinical Medicine, 2019, Volume 6, Issue 1, Pages 14-19

Aim: The aim of this study was to analyse the expression at the mRNA level of cytochromeb, subunits COI, COII, COIII of complex IV, ATP6 and ATP8 and to perform aquantitative analysis of the activity of ATP synthase as complex V of the respiratory chain, responsible for ATP synthesis and break down of ATP to ADP in breast cancer. Methods and Results: The level of gene expression at the mRNA level was evaluated on the basis of the light intensity of fluorochrome or fluorescein in the cells. Determination of the activity and location of the ATP synthase in cells was carried out using hybridization. In the formulations of breast cancer there was a higher genes expression at the mRNA level for all the examined genes as compared with non-cancerous tissue. The ATP activity was also higher in preparations obtained from breast cancer cells compared with the control tissue. Conclusions: The results confirm mtDNA incorporation to nDNA in neoplastic cells. They point to increased expression at mRNA level for COI, COII, COIII, ATP6 and ATP8 in breast cancer cells compared with control tissue. Increased amount of ATP synthase points to increased ATP in a neoplastic cell.