Online ISSN: 2515-8260

Keywords : Oxidative stress


EVALUATION OF ANTIOXIDANT ACTIVITY, TOXICITY STUDY, AND STANDARDIZATION OF RV FORTE CAPSULE

Dr. Vidhyadhar Vaidya

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 2852-2863

diabetes, metabolic disorders, atherosclerosis, and cardiovascular diseases. The incidence of such diseases is growing day by day around the globe and causes various complications. The disease not only affects the quality of life but also the mortality rate with this disease is too high. Considering these factors, it is important to come up with a simple yet potential solution for reducing oxidative stress. Therefore, RV New Visions Health Care Pvt. Ltd has developed an RV forte capsule as a potential treatment for reducing oxidative stress and help to fight against cancer.
Materials and Methods: Antioxidant activity of RV forte capsule was evaluated In-vitro by using DPPH scavenging assay, H2O2 radical scavenging assay, and Reducing power assay. Further, toxicity testing in animal models and the standardization using the HPTLC method was done.
Results: The formulation RV forte capsule showed significant anti-oxidant action when compared with standard ascorbic acid. The percentage scavenging activity of RV forte capsule was found to be 14.2 - 60.05 % at 50 - 500 ug/ml by DPPH scavenging assay,17.57 – 55.27 % by hydrogen radical scavenging assay, and the reducing power was found to be increasing with increased absorbance at 50-500 ug/ml concentrations. In toxicity testing, no toxicity was found at the dose of 100mg/kg.
Conclusion: The study thus proves, RV forte capsule has significant antioxidant activity

ROLE OF TORCH INFECTION, OXIDATIVE STRESS AND SOMATIC DNA DAMAGE IN HIGH-RISK PREGNANT WOMEN LEADING TO CONGENITAL ANOMALIES

Swapna C Senan; Anitha C; Sarala Gopalakrishnan; Dinesh Roy D

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 1125-1132
DOI: 10.31838/ejmcm.07.09.117

An increased complication rate during or after pregnancy and birth leads to a condition known to the mother or child when High Risk Pregnancy (HRP) and may be for both. An attempt was made here to find the role of TORCH infection in high-risk pregnant women; oxidative stress and somatic DNA damage, leading to congenital anomalies(CAs).The patients were referred from various infertility clinics and maternity centers of Kerala to Advanced Genetic Study Centre (Genetika), Trivandrum, Kerala. Study population includes 170 study subjects and 105 healthy controls. Blood sample was collected for TORCH IgG (ELISA), oxidative stress evaluation (Malondialdehyde by thibarbituric acid assay) and somatic DNA damage (CBMN assay). Among 275 subjects, 170 (61.81%) had the history of high-risk pregnancy with a mean age of 35.65±6.30 years. The control group included 105 women with previous normal pregnancy with a mean age of 34.03±6.10 years. TORCH infections had a significant role in HRP (p-value<0.001). Study group population showed a mean MDA value of 2.95 ± 2.09 and that of control group was1.67 ± 0.70. The mean value of CBMN frequency was 12.44 ± 1.01 in study subjects and that of controls was 9.98 ± 0.58. Study group showed a higher CBMN frequency and MDA value than control group. Proper diagnosis and intervention during early stage can be done by routinely screening of sample for TORCH agents, oxidative stress and somatic DNA damage even if the case is asymptomatic which shows high risk in postnatal periods. This can manage and reduce adverse foetal outcomes efficiently, thus reducing morbidity and mortality.

The Effect Of Extracts From The Mushroom Of The Ganoderma Lucidum On The State Of Oxidative Stress

Abilov Pulat Melisovich; Iriskulov Bakhtiyor Uktamovich; Boboeva Zuhra Nurillaevna; Saydalikhodjaeva Sayora Zamanovna; Yusupova Mokhira Tulyaganovna; Mirzaev Dilshod Akhmedovich

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 10, Pages 1814-1823

This article presents data on such a well-known fungus as Ganoderma Lucidum and its effect on the state of oxidative stress. As is known, under oxidative stress, altered Toll receptors cannot independently recognize microbial agents due to the altered chemical formula of L-galactosamine and thus mediators are not activated. It is also known that Ganoderma Lucidum due to β-polyglucans, which by chemical formula are close to this glycoprotein, can interact with leukocyte receptors and increase the adhesion molecules and leukocyte migration. Modern histological, ultrastructural and biochemical research methods are used. The results are presented and the corresponding conclusions are drawn.

Role of Polyphenolic Compounds in Management of Oxidative Stress Associated With Glaucoma

Chandrashekhar Mahadeo Chakole; Meenakshi Kanwar Chauhan

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 2064-2084

Glaucoma is a first rank common cause of irreversible vision loss. It is also
recognized as a neurodegenerative disease which progress with age, results in optic
neuropathy. The exact cause of glaucoma remains unclear although oxidative stress
considered as one of the reasons for cell death in the retinal ganglion cell and retinal
pigment epithelium. Oxidative stress could result after imbalance between formation and
utilization of reactive oxygen species. Current pharmacotherapy of glaucoma includes
lowering down of elevated level of IOP, which is not sufficient enough to retard
irreversible vision loss in some instances. Hence, alternative neuroprotective therapy is
warranted. Polyphenolic compounds possess antioxidant, anti-inflammatory properties and
also show the neuroprotective effect in an experimental model. Amongst the natural
polyphenolic compounds resveratrol, curcumin, rutin, quercetin, myricetin have been
studied and showed potential as neuroprotection against cell apoptosis. Moreover, the extra
supplement of a polyphenolic compound may also improve antioxidant status, which was
underestimated in glaucoma disorder. Despite the potential, the polyphenolic compounds
yet to explore for clinical use in ocular disorder. Hence it is an excellent opportunity for
the future researcher to transform these substances from lab to clinic as neuroprotectants
in glaucoma.

Night Jasmine (Nyctanthes arbortistis) having in vitro antimalarial activity from the leaves – a confirmation from ethnobotanical studies and traditional uses

Dr. Debasis Bisoi; Dr. Ashok Kumar Panigrahi; Dr. Lopamudra Das

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 11, Pages 6412-6423

Background: Night jasmine (Nyctanthes arbortristis or NAT) is a sacred plant (known as Parijat in Sanskrit) and commonly used traditional medicine. It was observed that the leaves of the plant are used in treating malaria. The present work has made an attempt to make ethnobotanical studies, isolation of the phytochemical constituents and mechanism of the pharmacological actions and therapeutic uses with focus on malaria. Objectives: (1) Identify the plant material for use in traditional medicine. (2)Gather laboratory evidence that the chemical constituents are comparable to standard treatment in malaria. Methods: The leaves of the plant (NAT)) were identified and the sample specimens were kept in herbarium after authentication. The phytoconstituents were identified by column chromatography. The antimalarial activities were studied. The in vitro activity of leaves of NAT was compared with chloroquine (CQ).

The Oxidative Effect Of Nlrp3 And Cyp2e1 In Development Of Renal Failure Associated With Hypertension And Diabetes Mellitus Diseases

Alyaa Abdulhadi Salih; Kareem Hamadx Kareem Hamad Ghali2

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 636-644

Renal Failure (RF) is devastating pathology with several causative factors, resulting in dialysis or kidney transplantation needed by end-stage renal disease (ESRD. The progression of RF is closely related to systemic oxidative stress, which causes various complications such as hypertension and diabetic mellitus . The current study was focusing on the oxidative effects of NLRP3 and CYP2E1 gene expression in the pathological development of renal failure disease with hypertension and diabetic mellitus as complications associated diseases. This study involved 100 patients diagnosed clinically and seriologicaly with renal failure 52 males and 48 females, their age from 17 to 80 years . Also the study comprise (18 males and 12 females) healthy volunteers as control group. RNA extracted from blood samples and converted to cDNA which subjected to real-time PCR for detection gene expression . The results detected that Hypertension and diabetic mellitus are the important risk factors that causes and advances renal failure disease . NLRP3 and CYP2E1 have significantly overexpression (1.60 folds and 5.37 folds ) respectively in RF patients compared with healthy group ( p <0.05). However, the expression of NLRP3 and CYP2E1 increase in diabetic mellitus (2.16 and 1.58 ) respectively than hypertension in RF patients with and control group (p<0.05). Diabetic mellitus generate free radical consequently causes ROS, hence elevated expression in oxidative NLRP3 and CYP2E1 genes in diabetic RF patients .

Sterically Hindered Phenols as Antioxidant

Mankar Viraj H; Chhavi .

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 3481-3491

Hindered phenolics are the compounds which will act as an antioxidant compared to the general phenolic compounds. Especially BHT compounds are shown here and which modifications will be responsible for the enhancing and reducing the efficacy of the phenolic compounds. These moieties are easily avaliable materials which found mostly in the foods, fruits and vegetables and also in several suppliments. The free radical oxidation of organic substrates is inhibited by phenols.and also involved in recovering from several pathophysiological conditions.In this review mentioned the several phenolic compounds which exhibits the effect of scavenging free radicals in the several pathophysiological conditions and daily life processes.

A Mini Review On Liver Injuries Among Humans Due To Drugs And Consumption Of Alcohol

Sumaira Yousuf; Mahendra P. Singh

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 4268-4276

Liver is the most important vital organ that carries out metabolism of body e.g. formation of cholesterol and triglycerides, synthesis of glycogen, formation of blood clotting proteins and bile formation. Hepatic damage and subsequent liver failure due to both unintentional and intentional acetaminophen overdose has afflicted patients for decades and includes the metabolic cornerstone pathways that exist within hepatocytes in the microsomes. The side effects of drugs on the hepatocytes have been overlooked for years. Drug induced liver injury is the most adverse effect observed in clinical practices. It accounts for around half of acute liver disorder. Anti tuberculosis drugs and acetaminophen overdose are the main issues that result in death from curable diseases. Metabolism of isoniazid and acetaminophen (APAP) results in the formation of toxic metabolites that results in the oxidative stress and glutathione (GSH) store depletion leads to hepatocellular necrosis and steatosis. Alcohol consumption is also the most common cause of liver cirrhosis. Liver peroxidation is considered to be the major mechanism of alcohol-induced hepatotoxicity. To overcome this oxidative stress, there is need of antioxidants that can balance the redox homeostasis of liver by lowering the APAP overdose effect. Consumption of natural antioxidants derived as secondary metabolites from plants in the form of polyphenols and flavonoids prevent oxidative stress. Medicinal plants are best remedies used for hepatotoxicity caused due to oxidative stress. They are cost friendly, easily available and have no mutagenic effect. Herbal medicines form important part of traditional healthcare system of India.

The role of oxidative stress on molybdenum enzymes and ischemic reperfusion injury in hyperuricaemic patients. An infrared spectroscopic study

Mamareli V; Tanis O; Anastassopoulou J; Kyriakidou M; Mamareli CH; Koui M; Theophanides T; Mamarelis I

European Journal of Molecular & Clinical Medicine, 2019, Volume 6, Issue 1, Pages 20-25

There are many clinical evidences that hyperuricemia is a risk factor for the development of peripheral carotid and coronary vascular diseases. However, the mechanism of elevated uric acid concentration in biological systems is not yet clear. In the present work Fourier transform infrared (FT-IR) spectroscopy was used to evaluate the mechanism of calcification and plaque formation in carotid arteries in hyperuricaemic patients. Comparison between the spectra of carotid arteries from patients with elevated uric acid values and spectra obtained from patients with normal uric acid values showed structural changes of the characteristic spectral bands in the region 4000-500 cm-1. These changes were related with changes in the concentration of the serum uric acid and the clinical history of the patients. The intensity decrease of the infrared bands in the region 1650-1500 cm-1 was associated with the decrease of the apolipoprotein ratio, ApoI/ApoII, which corresponds to HDL (High Density Lipoproteins) and the regulation of the LDL (Low Density Lipoproteins), which are related to oxidation stress. The infrared band at 1467 cm-1 indicated the presence of urea components as a result of the metabolic pathway. The shape and the intensity of the bands between 1250-900 cm-1 depend on the glycation-end products of the diseases. SEM-EDX chemical analysis showed fibril formation and molybdenum release in hyperuricaemic patients.