Online ISSN: 2515-8260

Keywords : Bacteremia

A Study of Pathogenic Bacteria Isolated from Blood Culture Samples and Their Antibiotic Sensitivity

Tiwari Palak, Mahi Ishani, Lal Darbari

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 5, Pages 517-525

Background: To diagnose bacteremia blood culture still remains the gold standard despite its limitations. While collecting samples, focus must be given that there is strong association between timing of specimen collection at different time points during admission and their yield.
Methods: A retrospective observational study was carried out by analyzing 100 positive blood cultures from April 2021-April2022. All positive blood culture and sensitivity reports of males and females aged less than one month to 60years were included. A total of 100 positive blood culture cases were taken from the culture and sensitivity register from Microbiology department of Hindu Rao Hospital and details were tabulated using a questionnaire.
Results: The commonest organism found on cultures was Coagulase Positive Staphylococcus (CONS) 34% of the cultures, this was followed by Actinobacter in 17% and Klebsiella pneumoniae in 11% of the culture samples. The most common fungi were Candida in 3% and Budding Yeast which was found in 2% of the samples. The most sensitive antibiotic was Gentamycin (27) which was sensitive for Coagulase-positive SA (12), K. pneumoniae (5), Actinobacter (5), S. typhi (3), E. coli (1) and P. aeuroginosa (1). The second most sensitive antibiotic was Vancomycin (20), which was sensitive for Coagulase-positive SA (9), Enterococcus (4), Methicillin resistant coagulase-positive SA (2), Methicillin sensitive SA (2), Actinobacter (1), MRSA (1), and S. aureus (1).
Conclusion: Positive blood culture is a crucial parameter for both the diagnosis of the patient as well as the associated prognosis, the correct interpretation of the blood culture results is essential. While planning treatment the sensitivity and resistance pattern of pathogens found in blood culture to common antimicrobial agents must be taken into account.


Venkata Sri Laxmi, Pravallika Mallipeddi, Varun Kanakam

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 4, Pages 1479-1484

Background:Neonatal septicemia is a clinical syndrome characterized by signs and symptoms of infection with accompanying bacteremia in the first month of life. Despite continuing advances in diagnosis and treatment, it remains one of the important causes of higher mortality and morbidity.Aim of our study is to evaluate the changes of platelet count and indices (MPV, PDW) in neonatal sepsis.
Materials and Methods: It is a prospective crossectional study conducted in Tertiary health center (PRATHIMA INSTITUTE OF MEDICAL SCIENCES) in the DEPARTMENT OF PATHOLOGY in colloboration with neonatology section over a time period of 7 months from January to August 2019.Using convenient sampling method, 100 neonates with sepsis and 100 normal neonates without any medical problems, as the control group were selected. Weight and gestational age matched healthy neonates without any infectious disease served as control. The groups were compared for age, WBC count, neutrophil count, platelet count, PDW AND MPV.
Results: In our study out of 100 cases, early onset sepsis was present in 30 cases, while late onset neonatal sepsis was present in 70 cases.40 neonates showed culture positivity and the remaining 60 showed culture negative sepsis. Statistically significant correlation of MPV with platelet count(high MPV in thrombocytopenic neonates) and outcome. Significant difference in PDW in thrombocytopenic neonates and non-thrombocytopenic neonates. Neonates with sepsis have significantly increased MPV, PDW compared to healthy neonates.
Conclusion: Platelet count and indices can be used as early diagnostic markers in neonatal sepsis. Neonates with sepsis have significantly increased MPV, PDW compared to healthy neonates. Platelet indices are inexpensive and easily available tests can be routinely performed for all neonates suspected to be in sepsis.

Prospective Study Of Isolates In Pyogenic Samples


European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 4052-4062

The advancement of specific automated systems in microbiology lab has increased the prognosis of treatment for various microbial diseases that were uncured during past. Various automated systems like VITEK 2, microscan make investigation criteria in simple and easy way such that microorganism specific for there biochemical tests are observed under computer operated device which makes the reporting quick and unbiased. In our medical center, we have diagnosed many patients with pyogenic infection[1]. Our aim was to study the isolates that were isolated from consecutive pyogenic samples coming in the hospital. We performed a prospective review of the records of the 70 unselected patients coming with pyogenic infection. We proceed the samples manually along with automated systems. Initial staining and culturing was done on BA, CA, MA which were later observed twice after 24hrs and 48hrs[2]. There was significant change in positivity of sample in case of difference in age and gender. According to the observations carried out by us that percentage of positivity increase with increase of age and with every male gender. Staphylococcus aureus was the the majorly identified organism in study of consecutive pyogenic samples[3]. Various anti toxins, enzymes, anti immune strategies of this organism were studied that are helpful for this bug to cause disease. The evaluations also included an assessment of risk factors, antimicrobial therapy and manual diagnosis methods. By Universal infection-control measures, the transmission of infections may be limited, patient education, screening and decolonization[4].