Online ISSN: 2515-8260

Keywords : Seropositive

Seroprevalence of Brucellosis Among Patient of Pyrexia of Unknown Origin at Tertiary Care Center of North-West Zone of Rajasthan

Bhagirath Ram Bishnoi, Yogendra Taneja, Yasmeen Bano, Abhishek Binnani, B P Sharma,Anjali Gupta,Geeta Tinna,Gautam Lunia

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 2203-2209

Introduction:Brucellosis, which is an important public health problem, is a zoonotic disease seen all over the world.
Aim: To detect the magnitude of problem of Brucellosis in this arid zone of Rajasthan.
Methods: This study was carried out on a total of 522 samples of suspected clinical cases between June 2019 to December 2019, at department of Microbiology, S.P Medical College, Bikaner. Serodiagnosis of brucella was done using serological tests and clinical findings.
Results: A total of 522 samples were screened and seroprevalence of Brucellosis was found 22.60%, maximum were in age group 0-15 year (39.83%), males (53.38%), rural (67.79%),animal owners (47.7%) followed by dairy farmers (22%) and minimum in butchers (0.84%). Most common clinical feature present was fever followed by headache and back pain.
Conclusion: Our study shows the high seroprevalence because agriculture activities, cattle rearing, and dairy farming were the main occupation in area of study.


Dr. Ajitha Reddy Edula,Dr. GJ Archana,Dr. Patruni Manoj

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 4, Pages 1961-1965

Introduction: Dengue is a mosquito-borne viral disease that has rapidly spread in all regions of WHO in recent years. Dengue virus is transmitted by female mosquitoes mainly of the species Aedes aegypti. These mosquitoes are also vectors of chikungunya, yellow fever and Zika viruses. Dengue causes a wide spectrum of disease. This can range from subclinical disease (people may not know they are even infected) to severe flu-like symptoms in those infected. Although less common, some people develop severe dengue, which can be any number of complications associated with severe bleeding, organ impairment or plasma leakage. Aims and objectives of this study are to know the prevalence of acute dengue viral infection in cases presenting with clinical features suggestive of Dengue through seroanalysis at Kamineni Hospital, Narketpally, Telangana.
Materials & Methods: This study is a hospital based cross-sectional study conducted in the year 2018-2019 in the Department of Microbiology,Kamineni Hospital, Narketpally, Telangana state from August 2018 to March 2019. A total of 120 blood samples collected from patients admitted in Kamineni Hospital with clinical features suggestive of Dengue fever. The serum samples were tested for IgM antibodies for dengue virus by dengue IgM capture ELISA. All the patients’ serum samples were tested for dengue IgM antibodies by IgM capture ELISA. This test is a solid phase immunoassay, based on an immunocapture principle.
Data analysis: The data was spread over excel sheet and the results were expressed as percentages and represented with tables wherever required.
Results: 72 were males and 48 were females, most of the cases were of the 10-20 age group. Most of the cases reported were from the young age groups.52 samples were positive for IgM antibodies to Dengue. More number of positive cases is among the males. The common clinical features among all the patients admitted was fever (100%) followed by headache (n=96, 80%), arthralgia (n=93,80%). The prevalence of dengue was found more among the rural population (n=33, 49.2%) when compared to the urban population (n=19, 35.8%).
Conclusion: The prevalence of dengue infection was more among 0-10yrs age group (48.2%).A significant association was observed between dengue seropositivity and the clinical presentation of the study population i.e., hepatosplenomegaly, conjunctival congestion, skin rash, retro bulbar pain and hemorrhagic manifestations.

Hepatitis C Seroprevalence Among A Tertiary Hospital Based General Population In Northern India

Heena Sharma; Vasim Mahdi Zaidi; Dr.Gomty Mahajan; Suman Kumari

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 4088-4094

Hepatitis C is a disease caused by enveloped, RNA virus belongs to Flaviviridae family. It causes inflammation of the liver that lead to liver cirrhosis and finally hepatocellular carcinoma. Its genome consists of open reading frame (ORF) codes for structural and non-structural proteins. The ORF have 5´ and 3´ UTR regions. Hepatitis C virus is cause of post transfusion hepatitis. Symptoms of HCV include fatigue, dark urine, belly pain, joint pain, itchy skin, sore muscles and jaundice. It is a blood-borne transmitted agent. Use of unsafe therapeutic injections also leads to HCV infection. Diagnosis of HCV infection can be done by various methods like Enzyme Immunoassay (EIA) and Recombinant Immunoblot Assay (RIBA). The aim of this study is to estimate seroprevalence of Hepatitis-C in both sexes and different age groups in hospital based general population. And to study the trends of HCV infections in a tertiary hospital located at Northern India.
A prospective study was conducted for four months (January-April) at Tertiary Hospital in Northern India. Total numbers of 1643 blood samples were screened for the presence of anti-HCV antibodies in patient’s serum. Samples were tested by HCV TRI-DOT rapid test. Positive samples were retested by SD BIOLINE HCV rapid test and confirmed by ELISA.Out of 1643, 102 (6.2%) samples were HCV positive. Among seropositive samples, 48 were males (2.9%) and 54 were females (3.2%). HCV seropositivity was shown by 40 IPD patients (2.4%), 52 OPD patients (3.1%) and 10 ICU patients (0.6%). Among departments, patients from Recovery showed (0.6%), General ward (1.0%), Private room (0.2%), Neurology lab (0.1%) and Emergency (0.3%) showed HCV seropositivity. On analyzing age-wise seropositivity, it was found that maximum seropositivity was seen in 30-40 years (2.9%) followed by >55 years (1.4%), 40-55 years (1.2%) and<30 years (0.5%). Analysis of patients on the basis of risk factors showed that 17 had history of surgical operation (1.0%), 18 had history of blood transfusion (1.0%), 6 had history of dental procedure (0.3%), 25 showed history of injecting drug use (1.5%), 21 used contaminated syringes (1.2%) and 15 patients was under haemodialysis (0.9%). Professional health worker should protect themselves while handling infected blood. Counselling and testing should be done for those who are at risk for infection.