European Journal of Molecular & Clinical Medicine

Background: Tendons are dense connective tissues composed primarily of a highly
organized type I collagen extracellular matrix (ECM). Which enables a tendon to perform its
mechanical function of force transfer. Platelet rich plasma (PRP) serves as a growth factor
agonist and has both mitogenic and chemotactic properties.
Aim: investigation the effect of PRP in tendon healing in rats, and evaluation the rate of
possible complications.
Materials and Methods: The study included (35) rats, (5) rats only used as donors for PRP,
while (30) rats separated as PRP group:(1); injection, group (2); spray, group (3); mixed.
The left leg in all rats (control group) receives nothing, while the right leg receives PRP as
separated groups. Each rat was kept in a separated cage and checked on until the end of the
follow up period (15-30) day. After (15) and (30) days post-treatment, (5) rats of each group
were sacrificed, and their Achilles tendons were extracted and examined histopathologically.
Stained sections examined for the number of the inflammatory cells / HPF,
the ratio between collagen 1 and collagen 3 and the arrangement of the collagen fibers

The purpose of our research was to assess the prognosis of the treatment performed using second generation platelet concentrates (PRF) and a new calcium silicate-based material (Biodentine) for coronal pulpotomy techniques.
Methodology: Pulpotomy procedure was carried out on 4 carious involved, permanent molars detected with acute irreversible pulpitis in 17- to 22-year-old patients. Taking into consideration the patient’s age and the condition of the underlying pulp tissue, PRF pulpotomy was planned in view of preserving the vitality of the intact radicular pulps. Regenerative procedures with second generation blood matrices were chosen to encourage
the recovery of the inflamed pulps. The follow-up examinations were conducted at 6, 12 and 24 months, revealing a better clinical and radiological result. Descriptive analysis was carried out to assess the prognosis all these teeth with subsequent follow ups.
Results: The clinical follow-up evaluation of the cases was met with a positive outcome. In addition, the digital radiographic examination (RVG) of the cases revealed an intact PDL space and a normal trabecular pattern of the bone. There was statistically significant dentinal barrier formation, which shows the healing reponse.
Conclusion: We can conclude that clinicians can safely rely upon advanced non-invasive, regenerative approaches to improve the standard of care delivered to the patients and can be considered as an alternative to conventional RCT.