European Journal of Molecular & Clinical Medicine

Background: Diabetes mellitus type 2 (DMT2) is one of the most prevalent endocrine
illnesses, affecting about 135 million individuals globally. The disease's genesis is
unknown, but subclinical hemochromatosis has recently been identified as one of the
possible causes of DM. The purpose of this study was to look at the association between
serum ferritin as a measure of iron excess and diabetes and HbA1c.
Methods: This 12-month study was carried out in the Medicine department of Katihar
Medical College Hospital in Bihar, India. 46 patients with T2DM were used as cases in
this case control study. The control group consisted of 46 healthy individuals who were
age, gender, BMI, and haemoglobin percent matched to the case group. Blood samples
were tested for ferritin, haemoglobin, HbA1c, and fasting plasma sugar. Anaemia and
any other ailment or medicine that could alter ferritin levels were exclusion criteria.
Result: The Pearson correlation coefficient test, Student's t-test, Chi-square test, and
Odds ratio were used to assess the results statistically. Diabetics had considerably
higher mean serum ferritin levels than the control group (197.97± 75.99 gm/L vs. 64.24±
27.83 gm/L, p<0.001). In diabetic patients, there was a substantial positive connection
between HbA1c and serum ferritin (p<0.001). In this investigation, an OR of 11.64 was
also discovered.
Conclusion: Serum ferritin levels are associated with T2DM patients. This could be a
significant and independent predictor of the development of diabetes mellitus

Background:Circular RNAs (circRNAs) are a class of non-coding RNAs (ncRNAs)
with covalently closed single-stranded structures. The epigenetic regulatory role of
ncRNAs in diabetes-associated cardiometabolic diseases is still evolving. This study
aimed to investigatewhether therelative expression level ofcirc. ankyrin repeat
domain36 (circANKRD36) are altered in Egyptian patients with T2DM and whether
they correlatedwith othercardiometabolic risk factors.
Methods: case-controlled study was conducted on forty patients with T2DM, and 20
healthy subjects were included in the study. Diagnosis of T2DM was based on ADA
Criteria.The expression profile of circANKRD36 was measured using quantitative realtime
(qRT) PCR.
Results: The relative expression levels of circANKRD36wassignificantly increased in
patients with T2DM compared to control. p˂0.001*.The relative expression levels of
circANKRD36 were significantly positive correlated with cardiometabolic risk
factorsand linear regression test revealed that hba1c as well as BMI, were
independently correlated with epigenetic markers. Our results revealed that the
sensitivities and the specificities ofcircANKRD36 expression levels to diagnosis of
T2DM were 87% and .99.15.
Conclusion: the relative expression levels of circANKRD36were significantlyincreased
in patients with T2DM.Also,the relative expression levels of circANKRD36 were
significantly positive correlated to cardiometabolic risk factors.