Keywords : Cytokeratin
"Utility Of Cytokeratin’s In Diagnosis Of Occult Lymph Node Metastases In Patients Of Squamous Cell Carcinoma Of Oral Cavity"
European Journal of Molecular & Clinical Medicine,
2020, Volume 7, Issue 11, Pages 3345-3354
Background: Squamous-Cell-Carcinoma of the oral cavity is the sixth utmost commonly ranked cancer globally. The mortality rates correspond to 2% of cancer deaths annually. Lymph nodes are the prime occurring site for metastasis of such cancers via infiltration of lymphatics. For clinically and radiologically undetectable metastatic deposits which is termed as occult metastasis, IHC acts as a gold standard modality for detecting micrometastasis and isolated tumor cells from resected lymph nodes. Cytokeratin present in the metastatic cells can be useful in detection of tumor cells.
Aim: To establish the utility of cytokeratin in diagnosis of occult lymph node metastasis in squamous-cell-carcinoma of oral cavity patients.
Materials & method: Prospective and analytical study was conducted on 100 SCC diagnosed patients in Histopathology and IHC section of Department of Pathology, Jawaharlal Nehru Medical College, Wardha, for 2 years. All biopsy confirmed cases with or without mets were taken after the routine processing and staining of the slides. Immunostaining with Cytokeratin AE1/AE3 was done for further analysis.
Results: Most commonly occurring site for oral cavity SCC was buccal mucosa. Out of 100 SCC patients, 54 came out to be positive for metastasis while 46 were negative for infiltration by malignant cells. IHC staining for such metastatically positive patients also showed cytokeratin positivity in 54 out of 100 such cases. Also 3 patients who were negative for metastasis came out to be cytokeratin positive.
Conclusion: H and E staining remained as the gold standard for diagnosis of while immunostaining for cytokeratin AE1/AE3 could also be used as an adjunct to the final diagnosis.