Online ISSN: 2515-8260

Keywords : CRP

A Prospective Analysis Of Diagnostic Accuracy Of High Sensitive C- Reactive Protein In Neonatal Sepsis

Shikha Muringathery Kakkat; Sham Lohiya

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 11, Pages 3780-3787

Background: Neonatal sepsis is an entity that involves blood stream infections associated with systemic symptoms in the first month after birth. Amongst the various types of sepsis in babies, it can be clinical which presents with characteristic symptoms, probable sepsis when clinical symptoms are also associated with positive lab findings and culture positive sepsis where in addition to the above blood culture would also be positive to infectious organism. Septicemia in a baby less than 28 days can also be classified into early and late onset depending on the time after birth that it presents. Early onset sepsis presents within 72 hours of birth and late onset presents after 72 hours of life. Diagnosing sepsis early is of prime importance to cut down the irrational use of higher antibiotics in culture negative neonates. This will not only curb drug resistant strains but also bring down the cost of intervention.
Aim: The main aim of our study is to measure the efficacy of high sensitive CRP (hs-CRP) in early diagnosis of neonatal sepsis.
Objectives: To determine diagnostic accuracy of hs-CRP in detecting neonatal sepsis.
Methodology: Ours will be a prospective cross sectional study where we will take two serial measurements of hs-CRP in clinically septic neonates and corelate the values with respect to other conventional screening markers like blood indices and CRP to determine its efficacy, sensitivity and specificity in detecting neonatal sepsis early. Blood culture will be taken as a gold standard to prove sepsis.
Expected Result: We expect hs-CRP to be more sensitive in detecting neonatal sepsis earlier than conventional sepsis screen of our center.
Conclusion: If proven effective in detecting sepsis early, this study could aid in the future incorporation of hs-CRP as part of routine screening of neonates for sepsis.

Using of calprotectin and lactoferrin levels in early detection of acute uncomplicated appendicitis in children

Athir Ahmed Kadium; Alaa Mohammed Ali Al Baazi; Mahmood Mosa Mahmood

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 4335-4341

This study was conducted in Kirkuk city for the period from December, 2019 to June 2020, and included 50 children with acute appendicitisand 50 children without any other disease, where the ages of children in both groups ranged from 3-12 years. The study included collecting information from children in both groups, including gender, age, living situation, number of family members, standard of living and social as well as the number of family members, number of rooms in the house. Amount of 5 ml of blood samples were collected from all the children included in this study, where the blood samples were separated by the centrifuge device and the serum samples were separated from them and kept in the refrigerator until the tests for determination of calprotectin and lactoferrin by ELISA and CRP by immunofluorescence. In this study, there were no significant difference between the 2 groups regarding age, sex and residence. The study found that, 95% of children with appendicitis was suffered from abdominal pain , 50% with fever and weight loss, 48% with nausea and 40% with vomiting. The study revealed a significant elevated level of calprotectin in children with appendicitis as compared with healthy children(18.54±4.25 vs 11.37±3.28 ng/ml) (P<0.001). The study also exposed a significant higher level of lactoferrin in children with appendicitis as compared with healthy children(10.51±1.52 vs 2.18±0.19 ng/ml) (P<0.001).The study showed that the maximum means of calprotectin and lactoferrin in children with appendicitis were observed in those with abdominal pain, followed by vomiting. The study revealed a significant positive correlation between calprotectin and lactoferrinlevel among children with acute appendicitis.The mean serum level of CRP was significantly elevated in children with acute appendicitiscompared to control group (27.57 ± 4.22 and 5.57 ± 3.9 mg/dl) respectively at a P value < 0.001. Conclusions:The study showed a significant relation of calprotectin and lactoferrin withappendicitis.