Online ISSN: 2515-8260

Keywords : biofilm

Insilico Interaction of Bioactive Compounds from Clove againstOral Candida albicans biofilm drug targets

Sindhu priya Kuppusamy; Lakshmi T; Surendar A

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 1, Pages 537-548

Aim: Insilico Interaction of Bioactive Compounds from Clove againstOral Candida albicans biofilm drug targets. Materials and methods: All the 3D models were obtained from PubMed and the final analysis was produced by two systems, mainly chemsketch and GOLD protein-ligand docking. Results : Here in this study we have tried to find the best compatible bio active compound of clove towards the target. Out of 5 Bioactive compounds from clove Eugenol acetate O4,O3 showed the highest docking score having h bond score to be 2.888(O4) , 2.653(O3). Conclusion: In most of the cases the docking the H bond value must be considered must be considered because the hydrogen bonds are stronger than van-der-walls bond and weaker than covalent bond as H bonds have the ability to create a bond or break a bond easily in this study we r trying to read the compatibility between the bioactive compounds and the target compound in this case its Candida albicans biofilm. Further the the research can be extended to wet lab work for further details.

Biofilm Formation And Quorum Sensing lasRGene Of Pseudomonas Aeruginosa Isolated From Patients With Post-Operative Wound Infections

Marwa Fotouh Mahmoud; Fayza Mahmoud Fathy; Maha Kamal Gohar; ael Mahmoud Awad; Manar Hassan Soliman

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 2, Pages 2177-2189

Background: Pseudomonas aeruginosa (P.aeruginosa) is one of the most commonbacteria capable of forming biofilms which are important in the establishment of P. aeruginosa infections on different host tissues includingpostoperative wounds. P. aeruginosa possesses at least two well-defined, interrelated QS systems, las and rhl that control the production of different virulence factors including biofilm development.Objectives: To determine the antimicrobial susceptibility pattern, biofilm producing ability and the presence of QS lasR gene in isolated P. aeruginosa strains from patients withpost-operative wound infections.Methodology:The study was conducted on Fifty-four clinical isolates of P. aeruginosa from infected postoperative wounds in patients admitted in Zagazig university hospitals, Egypt. Isolates from all patients were tested for antimicrobial susceptibility using
disk diffusion method, in vitro formation of biofilm in microtiter plates containing TryptoneSoya Broth (TSB) medium and detection of QS lasR gene using conventional PCR technique. Results: P. aeruginosa isolates showed high prevalence of resistance against ceftazidimeand aztreonam (74.1%), while they showed the lowest resistance to ceftolozan/tazobactam (7.4%). Biofilm formation was detected in 38 (70.4%) of P. aeruginosa isolates; 14.8%, 46.3% , 9.3% and 29.6% of isolates were strong, moderate, weak and non-biofilm producers, respectively. The lasR gene was detected in 42 (77.8%) of P. aeruginosa isolates. There was significant relation between biofilm formation and presence of lasR gene. Conclusion:Postoperative wound infection may serve as a reservoir for multidrug resistant biofilm forming P. aeruginosa. The QS lasR gene is strongly associated with biofilm formation,which can help in identifying lasR gene as a useful diagnostic marker for biofilm producing P. aeruginosa
strains isolated from infected wounds.