Online ISSN: 2515-8260

Keywords : CBC

Comparison of anemia characterization between automated CBC analysis and conventional peripheral blood smear assessment

Dr. Mehak Kashyap, Dr. Naveen Kakkar, Dr. Neelam Gupta

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 2, Pages 380-388

Introduction: Complete blood count (CBC) by the automated hematology analyzers and microscopic
examination of peripheral smears have traditionally been used in the diagnosis of anemias. The advent
of automated hematology analyzers has improved accuracy and precision of test results and has reduced
subjective errors. This study aimed at
i) Comparing anemia characterization between automated CBC analysis and conventional peripheral
blood smear assessment.
ii) Studying RBC histogram patterns in various categories of anemia.
Materials and methods: Blood samples from 500 adult anemic patients were run in Sysmex XP-100
fully automated, 3-part differential hematology analyzer. In all patients, blinded peripheral blood smear
examination by two observers was done. Anemia categorization by peripheral blood smears and
automated red cell data was compared.
Results: The number of patients with normocytic normochromic anemia on automated CBC was 280
and on peripheral smear examination was 269. On automated CBC, 137 patients had microcytic
hypochromic anemia whereas 107 patients had microcytic hypochromic picture on microscopy.
Significantly higher number (p<.05) of patients (76) with microcytic normochromic morphology was
diagnosed on blood smear compared to automated counts (17). When RBC volume and hemoglobin
content were considered together, a Kappa value of. 447 was obtained indicating moderate agreement
between the automated and manual (peripheral blood smear) assessment of anemia.
Conclusion: Patients with most anemia types can be accurately diagnosed by automated CBC analysis.
The peripheral blood smear has limitations in cases with borderline MCVs and mild hypochromia which
may be missed. It, however, still remains the cornerstone in the identification of abnormal RBC
morphology seen in hemolytic anemias.

Analysis of the Dengue Infection, Occurrence and Hematological Profile of Dengue Patients in Dhaka City

Mohammad Zakerin Abedin; Md. Sifat Uz Zaman; Tasnim Ahmad; Md. Abdullah Al Maruf; Md Babul Aktar; Md. Fayez Ahmed; Md. Abu Sayeed Imran; Rubait Hasan; Pinki Akter; Md. Ekhlas Uddin

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 1572-1578

In the tropical and subtropical area, dengue virus is mostly found. To the determination of the occur-rence of dengue viral infection and hematological profile of dengue infected peoples in the Dhaka city. A sum of 255 samples of blood from both sex and different age groups were collected in this research. Dengue nonstructural protein 1 (NS1) and complete blood count (CBC) test were performed for the study. During this study period, total 255 samples were tested. Among these, 67samples were diagnosed as positive. Seroprevalence of dengue was 26.30%. Out of 67 positive dengue patients, 41 (61.2 %) were male and 26 (38.8 %) were female. Dengue infection was observed more in 21 to 30years age group followed by above 40years and 16 to 20years but observed less in of 1st day to 5years followed by 11 -15years, then 6 -10years and 31 - 40years. It was seen that the >40years of age group had the maximum unit of cases having low platelet count followed by 21-30years and it was also seen that 21-30years of age group had the maximum range of cases having low WBC count followed by above 40years of age group. In our research, we showed thrombocytopenia (100000 - 150,000/μl) with leucopenia (White Blood Cells, WBC <5000/μl) in 8(26.6%) cases and thrombocytopenia (100000-150,000/μl) without leucopenia in 9 (24.3%) cases but thrombocytopenia (<100000/μl) with leucopenia (White Blood Cells (WBC) < 5000/μl) in 5(16.7%) cases and thrombocytopenia (<100000/μl) without leucopenia in 6 (16.2%) cases. Among 67 positive cases, we found that (26.6%) had DF and (16.7%) had DHF according to WHO classification. For validating more reliability, this research needs further work.