Online ISSN: 2515-8260

Keywords : Xylene


AN ECO-FRIENDLY ALTERNATIVE TO XYLENE FOR DEPARAFFINIZATION IN THE HEMATOXYLIN AND EOSIN PROCEDURE

Dr PREETHI, Dr.SAI SUDHA.M, Dr ANBUMOZHI

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 4, Pages 1031-1035

Introduction :
xylene is the widely used deparaffinizing agent in the Hematoxylin and Eosin procedure
because of its high solvent property. It is biohazardous in nature and causes toxic effects to
health. Hence there is need for the development of safer xylene alternatives in
histopathological procedures.
Aim :
The present study aims to assess the efficacy of eco-friendly Sweet lime juice as a
deparaffinizing agents in Hematoxylin & Eosin procedure and to compare it with routine
Hematoxylin & Eosin procedure using xylene as a deparaffinizing agent.
Materials and methods:
The present study includes 50 paraffin embedded blocks, two sections were prepared from
each block. One section( Group A) was stained with routine H & E stain with xylene and the
other section(Group B) was stained with xylene -free H &E stain with sweet lime juice as
deparaffinizing agent. Stained slides were examined by the pathologist and scores were given
to the staining parameters like cytoplasmic and nuclear staining, clarity, crispness and
uniformity of staining.
Results:
Staining parameters of all the group A and group B sections were examined and compared
with each other. Group B sections with sweet lime juice shows 85 % adequacy of staining for
the diagnosis.
Conclusion :
The results of the present study observed that the sweet lime juice can be used as safer and
eco-friendly substitute to xylene for deparaffinization in Hematoxylin and Eosin staining
procedure

AN ECO-FRIENDLY ALTERNATIVE TO XYLENE FOR DEPARAFFINIZATION IN THE HEMATOXYLIN AND EOSIN PROCEDURE.

Dr PREETHI, Dr.SAI SUDHA.M, Dr ANBUMOZHI

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 4, Pages 1310-1313

Introduction :
xylene is the widely used deparaffinizing agent in the Hematoxylin and Eosin procedure
because of its high solvent property. It is biohazardous in nature and causes toxic effects to
health. Hence there is need for the development of safer xylene alternatives in
histopathological procedures.
Aim :
The present study aims to assess the efficacy of eco-friendly Sweet lime juice as a
deparaffinizing agents in Hematoxylin & Eosin procedure and to compare it with routine
Hematoxylin & Eosin procedure using xylene as a deparaffinizing agent.
Materials and methods:
The present study includes 50 paraffin embedded blocks, two sections were prepared from
each block. One section( Group A) was stained with routine H & E stain with xylene and the
other section(Group B) was stained with xylene -free H &E stain with sweet lime juice as
deparaffinizing agent. Stained slides were examined by the pathologist and scores were given
to the staining parameters like cytoplasmic and nuclear staining, clarity, crispness and
uniformity of staining.
Results:
Staining parameters of all the group A and group B sections were examined and compared
with each other. Group B sections with sweet lime juice shows 85 % adequacy of staining for
the diagnosis.
Conclusion :
The results of the present study observed that the sweet lime juice can be used as safer and
eco-friendly substitute to xylene for deparaffinization in Hematoxylin and Eosin staining
procedure

An Ex-Vivo Stereomicroscopic Evaluation Of The Effectiveness Of Xylene, Endosolv –R And Eucalyptol Along With H-File In Retrieving The Gutta-Percha And Ah Plus Sealer From The Root Canal

Annu Mayank; Atul Jain; Rachana Bahuguna; Abhinay Agarwal; Rohit Sharma; Gopi kumhar

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 8, Pages 4647-4655

Aim: The aim of this ex-vivo stereomicroscopic study was to compare the dissolution
efficacy of Xylene, Endosolv-R and Eucalyptol along with H-file in retrieving Gutta-
Percha and AH Plus sealer from the obturated root canal. Materials and Methods: A total
of 30 freshly extracted lower permanent mandibular first premolars were obturated and the
samples were placed in incubator to maintain 100% humidity at 37℃ for 7 days. After a
week, samples were decoronated to standardize the working length (15mm from apices to
coronal end). Reservoir of 3-4 mm was created inside each canal and samples were divided
equally into three groups with Xylene (group 1=10), Endosolv (group 2=10) and
Eucalyptol (group 3=10). 1 ml of each solvent was dispensed in each tested sample and
waited for 2 minutes, retrieval was carried out using 25# H-file. After radiographic
verification, longitudinal splitting of each sample was done with mandrel and disc. The
images were visualized under stereomicroscope at 20x magnification to access the presence
and absence of filling materials along the entire sections, photographs were captured using
digital camera and images were transferred to Digimizer 4.5 version for evaluation of the
area devoid of obturating materials. The collected datas were assembled for further
statistical evaluation using ANOVA and Tukey HSD test. Results: On evaluation, all the
tested agents, produced certain degree of dissolution. On comparing the mean values
between Xylene, Endosolv-R and Eucalyptol in coronal portion, it was found to be 10.763,
7.143 and 5.94, in middle third the mean obtained was 9.526, 5.408 and 5.906 whereas in
apical third the values were 9.334, 6.002 and 6.123 respectively. Conclusions: The results
of our study showed statistically higher dissolution capacity with Xylene when compared to
Endosolv-R and Eucalyptol, whereas, no significant difference was noted between the
dissolving capacity of Endosolv-R and Eucalyptol group. The mean obturation loss in
coronal, middle and apical third with Xylene was found to be highest when compared with
others. On comparison between Endosolv-R and Eucalyptol, the mean area loss with
Endosolv was found to be higher in coronal third, whereas, Eucalyptol was more effective
in dissolving the materials in middle and apical third region.