Online ISSN: 2515-8260

Keywords : monocytes


Huldani .; Ahmad Husairi; Abdullah Zuhair; Muhammad Rafagih; Zhasifa Khoirunnisa Suwanto; Wisnu Wiryawan; Muhammad Zaini

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 1, Pages 1509-1516

This study aims to mention the difference in VO2 max value in adolescents and the difference in leukocyte,
monocytes, and neutrophil levels after 12 minutes of moderate aerobic exercise. The study was conducted using a cross-sectional study in 15 basketball players & 15 non-basketball students at SMAN 1 Banjarbaru. The sampling technique was carried out using purposive sampling method. Measurement of VO2 max value was carried out on the first day using the MFT (Multistage Fitness Test / Bleep Test) and measurement of leukocyte levels was carried out on the third day using blood collection after the patient did 12 minutes of moderate aerobic exercise. Data analysis used the Mann-Whitney test for VO2 max values & unpaired t test for the number of leucocytes, monocytes, neutrophils & cortisol levels. The results of the data analysis showed that there were still significant differences in
the VO2 max value in basketball & non-basketball players (p> 0.05), monocytes (p> 0.05), & neutrophils (p> 0.05) and there were significant differences in levels. cortisol (p <0.01) after 12 minutes of moderate aerobic exercise in basketball and non-basketball students. The conclusion of this study is that 12 minutes of moderate aerobic exercise resulted in a higher increase in cortisol levels in basketball players compared to non-basketball players and the average value of leukocytes, monocytes, and neutrophils in basketball players was lower than non-basketball players. This is because the release of cortisol is a form of adaptation to the body's stress due to training, and becomes anti-inflammatory as a result reduces the number of leukocytes, monocytes and neutrophils of basketball players. 12 minutes of aerobic exercise can be used as a method to increase the body's immune system and explain the homeostasis process in adolescents in a good way.

Differential Recruitment of Monocytes Subsets in Chronic Hepatitis C Patients

Nora E. El-Bassiouni; Mona E. Madkour; Raafat I. Atta; Mohamed D. El Talkawy; Azza M. El Amir; Alyaa A. Farid; Noha A. Amin

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 11, Pages 6071-6082

Background: Collagen producing myofibroblast activation of is critical for pathogenesis of liver fibrosis. Aim of the work: To study the expansion of peripheral monocyte subsets in HCV patients. Subjects and Methods: Sixty HCV patients were classified according to METAVIR score into 4 stages of liver fibrosis, 15 age and sex-matched controls were include. Flowcytometric analysis of peripheral blood monocytes subsets and CCR2+ve cells was
carried out using monoclonal anti-CD45, anti-CD14, anti-CD16, anti-collagen type I and anti-CCR2antibodies. MCP-1 and SAP levels were assessed using ELISA. Results and Conclusions: A down regulation (p< 0.01) in the classical monocytes subset and an up regulation (p< 0.01) in both the non-classical monocytes and monocytes
producing collagen subsets were notice in HCV patients compared to controls. A marked increase (p< 0.01) in the levels of MCP-1 and monocytes expressing CCR2 with a significant decrease (p< 0.01) in SAP levels, which paralleled the progression of liver fibrosis, were also noticed. MCP-1 and CCR2 may trigger monocytes recruitment to the injured liver promoting the development of collagen type I producing monocytes. The shift
of classical monocyte subset towards the non-classical and collagen producing subsets may be present a predictive non-invasive biomarkers for progress of liver fibrosis.