Online ISSN: 2515-8260

Keywords : acute kidney injury


Urinary Tissue Inhibitor of Metalloproteinases-2 as Early Biomarker of Acute Kidney Injury after Cardiac Surgery

Esam El–Din Mahmoud Lotfy Omar MD, Adel Abd El-Mohsen Ghorab MD, Heba S. Abdel-Aziz Assal MD, LamiaaAbd El-Wahab Mohamed, Emad A. William, Maii Mahmoud Ahmed Temraz

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 3759-3770

Background: Tissue inhibitor of metalloproteinase-2 (TIMP-2) is a member of the
matrix metalloproteinase family, inducer of G1 cell cycle arrest, it is a marker of
cellular stress in the early phase of tubular cell injury caused by a wide variety of
insults
Aim and objectives: The aim of this work was to study if urinary TIMP-2 can be used
as one of early biomarkers of acute kidney injury after cardiac surgery.
Subjects and Methods: This was cross sectional study was conducted in collaboration
between the Internal Medicine, Cardiothorathic and Clinical pathology departments,
Faculty of Medicine, Zagazig University Hospitals. A total number of 50 patients
were included and classified into two main groups: Group I : included 25 patients
who undergone coronary artery bypass graft. Group II: included 25 patients who
undergone valve replacement surgery. The patients were reclassified after the
procedure into two groups: AKI group: defined on 24 h creatinine level elevation
either by 25% of the basal level or by 0.3 mg/dl above the basal level. No AKI group:
No rise of the serum creatinine level after 24 hours of the operation. The duration of
the study ranged from12- 18 months.

Renal Protective Effect Of Vitamin D3 In Isoproterenol-Induced Myocardial Infarction In Rats

Akmal Ahmed Hassan Diab, MD; Khaled Abdelfattah Abulfadle; MD; Nourelhuda A. Mohammed; MD and Fatma NabilHashim

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 2, Pages 1341-1357

Acute kidney injury is a common complication of myocardial infarction (MI) and scarce data were available on the effect of vitamin D3 on heart and kidney functions in MI. In this study, we investigated the potential protective effect of vitamin D3 on cardiorenal functions in isoproterenol-induced MI in rats and the possible mechanisms involved. It was shown that in MI rats, there was a significant increase in serum levels of (creatine kinase myoglobin binding, lactate dehydrogenase, creatinine, malondialdehyde, interlukin-6 and tumor necrosis factor alpha) and urine levels of (total protein and albumin), with a significant decrease in urine creatinine level, creatinine clearance and serum levels of [reduced glutathione and 1, 25 dihydroxy vitamin D] in comparison with
the control rats. On treatment of rats with vitamin D3 prior to induction of MI, thesebchanges were significantly improved in comparison with the MI rats. Histopathologicalband immunohistochemical examinations of heart and kidney in MI rats reflected the deterioration in their structures with presence of marked apoptosis which were ameliorated on treatment of rats with vitamin D3 prior to induction of MI. In conclusion, vitamin D has a protective effect on heart and kidney functions in the rat model of myocardial infarction and this beneficial effect could be related to its anti-inflammatory, antioxidant and anti-apoptoticactions.

Renal Protective Effect of Vitamin D3 in Isoproterenol-Induced Myocardial Infarction in Rats.

Akmal Ahmed Hassan Diab, MD; Khaled AbdelfattahAbulfadle, MD; Nourelhuda A. Mohammed, MD; Fatma NabilHashim

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 2, Pages 1928-1944

Acute kidney injury is a common complication of myocardial infarction (MI) and
scarce data were available on the effect of vitamin D3 on heart and kidney functions in MI. In
this study, we investigated the potential protective effect of vitamin D3 on cardiorenal
functions in isoproterenol-induced MI in rats and the possible mechanisms involved. It was
shown that in MI rats, there was a significant increase in serum levels of (creatine kinase
myoglobin binding, lactate dehydrogenase, creatinine, malondialdehyde, interlukin-6 and
tumor necrosis factor alpha) and urine levels of (total protein and albumin), with a significant
decrease in urine creatinine level, creatinine clearance and serum levels of [reduced
glutathione and 1, 25 dihydroxy vitamin D] in comparison with the control rats. On treatment
of rats with vitamin D3 prior to induction of MI, these changes were significantly improved in
comparison with the MI rats. Histopathological and immunohistochemical examinations of
heart and kidney in MI rats reflected the deterioration in their structures with presence of
marked apoptosis which were ameliorated on treatment of rats with vitamin D3 prior to
induction of MI. In conclusion, vitamin D3 has a protective effect on heart and kidney
functions in the rat model of myocardial infarction and this beneficial effect could be related
to its anti-inflammatory, antioxidant and anti-apoptoticactions