Online ISSN: 2515-8260

Keywords : Wolbachia

Detection and phylogeny of Wolbachia in field-collected Aedes albopictus and Aedes aegypti from Manila City, Philippines

Maria Angenica F. Regilme, Tatsuya Inukai, and Kozo Watanabe .

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 3060-3073

Wolbachia is the most common bacterial endosymbiont of arthropods, such as the medically important Aedes albopictus. Recent reports also detected in Aedes aegypti. This study collected 12 adults Ae. albopictus and 359 Ae. aegypti from 183 households in a dengue-prone area, Manila, Philippines, between June and September 2017. Aedes larvae (n = 509) were also collected from 17 water containers from 11 households. The DNA of the Aedes larvae and adults were screened for the presence of Wolbachia using the wsp and 16S markers, following optimized polymerase chain reaction (PCR) conditions, and sequenced. Our results showed that 12 out of 12 (100%) adult Ae. albopictus and3 out of 359 (0.84%) adult Ae. aegypti were Wolbachia positive, whereas all larvae tested negative for Wolbachia (0/509; 0%). The wsp marker revealed six Wolbachia-positive Ae. albopictus belonging to supergroups A (n = 2) and B (n = 4). The 16S marker showed Wolbachia in ten Ae. albopictus and 3 Ae. albipictus,most sequences were in supergroup A (n = 11) and two in supergroup B. Our results revealed Wolbachia supergroups in field-collected Ae. albopictus and Ae. aegypti with implications for a successful Wolbachia mass release programs.


Savira Ekawardhani; Imam Megantara; Arifudin Achmad; Hadyana Sukandar; Lia Faridah

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 2, Pages 1989-1997

A new alternative in controlling mosquitos, the arbovirus vectors, has emerged in recent years and commonly called Wolbachia method. Currently, a large trial is still running since 2017 in Indonesia to assess the efficacy of Wolbachiainfected mosquito deployment in reducing dengue incidence in Yogyakarta. Awaiting the trial result before implementing the same method in Bandung, however we could not find any reports on the distribution of natural Wolbachia in urban mosquitos from Indonesia, especially for Culex sp and Aedes sp of Bandung. Therefore, field surveys were conducted at four locations (3 subdistricts) from Bandung City and its surrounding area to screen for the natural mosquito infection status of Wolbachia by PCR and sequencing method. We
found that 83.3% of Bandung-captured Cx. quinquefasciatus were female, and 88.2% of those female were naturally infected with Wolbachia. The infection rate was lower for the male Culex, bringing down the total infection rate of Cx. quinquefasciatus to be around half of the captured mosquitos. However, the results from both female Ae. Aegypti of different locations revealed baffling very weak bands on the agarose gel. As for the infection status of Bandung’s captured Ae. aegypti, it is worth to wait for our further results as well as reports
from other studies. And thus, we opted to keep the inconclusive status of Wolbachia infection in Ae. aegypti from Bandung City