Online ISSN: 2515-8260

Keywords : nystatin

Chitosan – A naturally occurring viable alternative to Nystatin as an anti-fungal agent incorporated in heat polymerised denture base resin

Trunaya Bhatlekar; Siddharth Swarup; Swapnil Kurhade; Manish Shivaji Jadhav; Dhananjay Gandage; Amit Jagtap

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 11, Pages 5904-5915

Aims and Objectives: To evaluate and compare the antifungal efficacy of low molecular weight chitosan and nystatin by their inhibitory action on candida biofilm formation on Triplex heat cure denture base after 7 days and 14 days. Materials and Methods: A total of 10 specimens were fabricated using the Triplex heat cure PMMA acrylic resin by the conventional compression molding technique.Candida albicans biofilm was allowed to form over a period of 72 hours on all the specimens after pre-treatment with artificial saliva. Two groups of 5 specimens each were established. Group A was treated with low molecular weight chitosan suspension and group B was treated with nystatin suspension, both on the 4th day after biofilm formation. The treated specimens were now stored in a yeast nitrogen base broth medium to complete the time period of 7 days for initial maturation of the biofilm. The CFU count of candida colonies was recorded on the 7th day in both the groups.