Online ISSN: 2515-8260

Keywords : Skin Prick Test

Pattern of Aeroallergen Sensitization in Children with Respiratory Allergy in North Indian Population

Radhey Shyam Sharma, Pratibha Shekhawat, Pooja Yadav, Gautam Lunia

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 5755-5760

Background: Allergic diseases affects lives of more than one billion people worldwide(1).
Aeroallergen sensitization can be evaluated using either skin testing or measuring
specific IgE to these aeroallergens. Skin prick testing (SPT) is an easy, cost-effective and
convenient approachto identify sensitization to allergens.
Objective: To determine the pattern of sensitization to various aeroallergens and
prevalence of atopy in children with respiratory allergy.
Method: We performed a hospital based prospective observational study, conducted at
Paediatric OPD, SantokbaDurlabhji Memorial Hospital, Jaipur, India. Total 140
children of age 6years to 18 years diagnosed with Respiratory allergy (Allergic rhinitis
& Asthma) were enrolled. Skin prick test using 18 aeroallergens(indoor & outdoor)
were performed as per standard protocol.
Result: Among the total cases around 77% (n=108) were having positive skin test for
one or more allergens, most common allergens were HDM (35%), cockroach
(32.2%),Mosquito (32.1%), Grain dust (22.1%) and Heloptelia (20%).
Conclusion: SPT is the most convenient, cost-effective, sensitive and standardized
method for detecting allergen sensitivity and should be used in all children with allergic
disorders to demonstrate the atopy. More than 50% of children in our study had
sensitization to one or more aeroallergens suggesting atopy; sensitization was most
commonly seen to HDM antigen.

Allergic Rhinitis in Relation to Gut Microbiota Composition among School-Aged Patients

Samir Sourour, Mahmoud Diab, Rania M. Amer , Tarek Abdelmouty, Gehan A. Elshenawy, Saeid Abdelmonaem

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 4, Pages 457-465

Background: Allergic rhinitis (AR) is one of the most globally commondiseases and
usually persists throughout life. First-line drugs can be successfully used to control
AR. However, once these medications are terminated, the majority of AR patients
will reappear the symptoms of AR within a brief period. Thus, these medications do
not appear to exert a long-term effect on the baseline Total Nasal Symptom
Score.To study the relationship between the gut microbiota types and allergic
rhinitis. Patients and Methods: This case control study was carried out on 52
school-aged patients presented at Oto-rhino-laryngology, Head and Neck (ORLHNS)
Department and Microbiology and Immunology department at Zagazig
University. The patients were classified into 2 groups: Allergic rhinitis group: 26
individuals with allergic rhinitis Control group: 26 individuals without allergic
rhinitis. All patients in this study were subjected to the personal history taking,
physical examination and laboratory investigations including: eosinophilic count,
skin prick tests (SPT) for common perennial and seasonal allergens, measurement
of (total Ig E) and identifing bacteria (lactobacillus and bacteroides) by SYBR
Green real time PCR. Results: Age was distributed as 11.19±3.26 and 11.65±3.24
respectively between case and control with no significant difference between
groups. Mean disease duration was 6.15±2.01, the majority were Intermittent and
mild regard classification also the majority were trigger by inhalation. Skin prick
test among cases group showed majority were house dust and date palm pollens.
Absolute eosinophils counting was significantly higher among cases compared to
control one. Cases were significantly higher regard IgE distribution. Lactic acid
bacteria RNA was significantly higher among control group compared to cases
group while Bacteroid bacteria RNA was significantly higher among cases group
compared to control group. Conclusion: Lactic acid bacteria in gut microbiota of
school-aged individuals may influence sensitization to different allergens.

IgE Reactivity to Latex in sera of Indian Patients: An Original Research

Dr. Heena Tiwari; Dr. Harshada Inamdar; Dr. Rahul VC Tiwari; Dr. Anil Managutti; Dr Rakhi Bharat; Dr. J. Sreedevi

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 11, Pages 5936-5941

Aim: The purpose of our research was to assess and analyse IgE cross-reactivity in Indian patients sera in relation to latex extract.
Methodology: Sera from 27patients with latex allergy and control sera from nonsensitive individuals were studied for latex-specific IgE antibodies. Two antigen preparations were used from different rubber tree saps. All patients had skin prick test results that were positive to latex antigens, and all sera were evaluated by enzyme-linked immunosorbent assay (ELISA) with the various antigens.
Results: There were considerable differences in the reactivity of patient sera with the different antigens. Only 50% of the sera from patients with later allergy demonstrated significant levels of IgE to later as determined by enzyme-linked immunosorbent assay.
Conclusion: The results indicate that reagents such as rubber tree sap, which contain multiple clinically significant antigenic components, should be included in evaluation of latex allergy and that differences in patient populations may result in serologic variances