Online ISSN: 2515-8260

Keywords : CIN

Immunohistochemical Evaluation of Preneoplastic and Neoplastic Lesions of Cervix using p16INK4A and MIB-1

Dr.Prashant singh, Dr. shikha prakash, Dr. Lalit kumar, Dr. Nupur K, Dr. Ruchika S

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 7, Pages 579-593

Introduction:Cervical cancer is fourth most common cancer in women and account for about 5% of cancer deaths in females’ worldwide. In India, cervical cancer ranks the first most frequent cancer among females between 15 and 44 years of age. About 99% cervical cancersare linked with Human papilloma virus (HPV).
Aim:To evaluate the immunohistochemical expression of p16/INK4A and MIB-1 in preneoplastic and neoplastic lesions of cervix.
Material and Methods:
The cross sectional study was conducted on 63 cases in department of pathology of our institute over a period of two year from Dec 2018 to Dec 2020. The specimens were processed and three sections of 2-3 micron thickness were made and one stained with H&E stain, examined and 63 cases were selected. Other two sections were used for immunohistochemical staining
Result:In the present study, out of 63 cases, maximum 40 (63.49%) cases were observed in 31-50 years age group. In this study on P16INK4a immunostaining, maximum cases (8/15) of CIN-I and (9/15) of CIN-II histology were found grade-2 and grade-3 positivity respectively. Maximum (2/3) cases of CIN-II and all cases of squamous cell carcinoma histology showed grade-3 positivity. In this study onMIB-1 (Ki67) immunostaining, Maximum (12/15) cases of CIN-I were found grade-1 positive. All cases of CIN-II, III and carcinoma cervixes were positive.
Conclusion: We concluded that p16 INK4A and MIB-1 over expression was observed in CIN I/LSIL, CIN II/HSIL, CIN III/HSIL. Significant up regulation of P16 INK4A and MIB-1 was observed in carcinoma cervix.

The Predictive Value of Oral Nicorandil on Contrast Induced Nephropathy in Patients with Renal Insufficiency Undergoing Cardiac Catheterization in Non ST Segment Elevation Acute Coronary Syndrome

Ayman Ahmed Seleim Msc; Osama Mohamed Hassan MD; Ahmed Shawky El-serafy MD.; Ahmed Ibrahim El-Desoky MD

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 331-351

CIN leads to increased morbidity, prolonged hospital stay and thereby, more health care costs. The incidence of CIN varies from 2% to 30%. Fortunately, most cases can be completely reversed within two to four weeks. The optimal therapeutics used to prevent and CIN remains unclear. The main objective of the current study is to assess the effect of oral Nicorandil on the occurrence of CIN in patients with renal insufficiency undergoing cardiac catheterization in NSTEACS