Online ISSN: 2515-8260

Keywords : DPPH


IN VIVO AND in VITRO ANALYSES to REVEAL THE POTENTIAL of SOLANUM VIOLACEUM as EFFICIENT HEPATOPROTECTIVE AGENT

Remya K; Balamurali M M

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 41-58

Objectives The antioxidant and hepatoprotective activity of Solanum violaceum was evaluated with carbon tetrachloride (CCl4) intoxicated animal models followed by phytochemical screening of bioactive extracts by GC-MS. Methods The total phenolic and flavonoid content were estimated from the solvent extracted samples. In vitro antioxidant and in vivo hepatoprotective studies were carried out with the ethyl acetate extract of Solanum violaceum (SVEE). The hepatoprotective evaluations were carried out with CCl4 intoxicated rats. The levels of Serum Glutamate Oxaloacetate Transaminase (SGOT), Serum Glutamate Pyruvate Transaminase (SGPT), Serum Alkaline Phosphatase (ALP), bilirubin and proteins were measured in the serum and histopathological studies were carried out with the liver sections. The phytochemical profile of the bioactive extract was revealed from GC-MS analysis. Results The ethyl acetate and chloroform extracts of Solanum violaceum were rich in phenolic and flavonoid contents respectively. The DPPH and NO scavenging assays revealed better activity for ethyl acetate extract while ABTS assay was better for the alcoholic extract. Therefore further investigations on the hepatoprotective activity were carried out with ethyl acetate extract. The hepatoprotective screening revealed the restoration of enzymes as well as protein to normal levels upon treating intoxicated rats with the bioactive extracts in a dose dependant manner. Further histopathological studies also revealed that the ethyl acetate extract was effective towards restoration of liver cells to normal levels. The phytochemical profile as evaluated from the GC-MS analysis of the extract narrowed down the observed response to four major compounds. Conclusions Solanum violaceum possesses significant antioxidant and hepatoprotective activities. The bioactive constituents attributing to the observed activity were revealed by GC-MS analysis. Viridiflorol, palmitic acid, n-pentacosanal and citroflex A were found to be the major ones

Estimation Of Phenolic Compounds And Evaluation Of Their Antioxidant Activity Of Some Parts Of The Orange Plant (Citrus Sinensis L.)

Iman Mohsin Kadhom; Nagham Saddon Ibrahim; Najim Abdullah Al-zubaidy

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 4811-4822

Orange fruits were collected from one of the gardens of Baqubah / Diyala / Iraq in January of 2020, while samples of leaves and flowers were collected in March of the same year, for the purpose of completing the research procedures. The present study aims to estimation of phenolic compounds and evaluation of their antioxidant activity of the extract of some parts of the orange plant. The results show a variation in the parts of the orange plant in their content of phenolic compounds. Moreover, the statistical analysis pointed to the presence of significant differences at the probability level of P <0.05 among the parts of the orange plant, in Total Phenolic Content (TPC) and Total Flavonoid Content (TFC). The leaves are superior in their (TPC) which record the highest in comparison with the juice, which recorded the 1-concentration of 145.71 mg( GAE) .g. Though the highest (TFC) in the mesocarp of fruit is 1-lowest (TPC) 57.18 mg( GAE) .gin the juice, which gave the 1-compared to 5.81 mg.Querecetin. g 1-mg Querecetin .g 11.14lowest (TFC).
On the other hand, the result of statistical analysis showed significant difference among the parts of orange plant at the probability of P <0.05 in antioxidant activity. The result showed that the highest antioxidant activity, detected by using the DPPH scavenging . While the lowest value of the free radicals DPPH 1-test of the flowers is 2.64 mg. mLwas obtained from exocarp. The statistical analysis 1-inhibition, which was 0.86 mg. mLresults indicated a positive correlation among the (TPC), (TFC) and the antioxidant activity of DPPH free radical test with value of 0.385 and 0.484 respectively.