Online ISSN: 2515-8260

Keywords : Antibiotic


The Role of Antibiotic Prophylaxis in Port-Site Infection in Laparoscopic Cholecystectomy

Dr.Servishet Saraf, Dr.Suhail Masood, Dr.Monika Bhagatl, Dr.Neeraj koult

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 1934-1939

Background: Laparoscopic surgery has rapidly gained wide spread acceptance for the
last couple of decades, yet it is marred with the occurrence of post-operative port site
infections. One of the better ways of tackling port site infection is its prevention. Hence
the present study was conducted to compare the efficacy of single dose antibiotic
prophylaxis vis conventional multidose antibiotic therapy in patients of low risk elective
laparoscopic cholecystectomy.
Materials and Methods: The patients were divided in two groups, Group A and Group
B with 34 and 36 patients respectively. Patients were assessed for any post-operative
fever at frequent intervals for 24-48 hours and noted down and port sites were assessed
for any induration, discharge, erythema or tenderness.
Results: The age of the patients ranged from 19-55 years and 15-55 years in group A
and group B respectively. Three patients in each group developed low grade fever in the
post-operative period (day 1) that settled with paracetamol and didn’t merit further
evaluation. Two patients developed port site discharge with mild gaping at the
epigastric port site in group A. One patient developed port site discharge in group B.
The difference was statistically insignificant.
Conclusion: Hence, it is concluded that the efficacy of the single dose antibiotic
prophylaxis is the same as the conventional multiple dose antibiotic prophylaxis
therapy.

Antibiotic Resistance Among Uropathogenic Escherichia Coli

Dr. Aarti Akhand, Dr. Ramanath Karicheri

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 4914-4919

Background & Method: The study is conducted with an aim to study Antibiotic resistance among Uropathogenic Escherichia Coli, targeted both male and female outpatients and inpatients presenting with symptoms and signs of UTI which include dysuria, polyuria, fever, nausea, and flank pain were sampled for this study. This study is done with an aim to study Antibiotic resistance among Uropathogenic Escherichia Coli. Baseline demographic data including age, sex, level of education and risk factors such as catheterization, history of UTI, also out and in patients were also collected.
Result: Among the total 120 isolates, resistant to ≥2 drugs were recorded in 108 (90 %) of all uropathogens. Seventy seven (93.9 %) isolates of Gram-negative bacteria and 31(81.6 %) of Gram positive bacteria showed resistance to two or more drugs (Table 4). Resistance pattern of bacterial isolates to more than two antibiotics of patients (N = 120)
Conclusion: There is a need for continuous surveillance of antibiotic to the currently used antibiotics in management of urinary tract infections covering the entire Index Hospital . Index Hospital to enforce policies formulated by pharmacy and poison board to prevent misuse or underuse of antibiotics by giving prescription to only patients with results of culture and sensitivity and therefore treatment UTIs should be based on and sensitivity in order to limit multidrug resistance. Continuous follow up to provide an update of laboratory diagnosis of urinary tract infections in order to reduce multidrug resistance bacteria in UTI patients. Health care workers should enforce health education to patients in order to adhere to the treatment and thereby reducing drug resistance. v) Screening for resistance and identify modes of transmission.

DETECTION of SOME VIRULENCE FACTORS and ANTIBIOTIC SENSITIVITY TEST of ENTEROCOCCUS FAECALIS ISOLATED FROM SHEEP by MULTIPLEX PCR

Hala Mohammed Majeed; Bashar Sadeq Noomi; Marwan Q. AL-Samarraie

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 68-74

Enterococcus faecalis form an important population of commensal bacteria and have been reported to possess numerous virulence factors considered significantly important in exacerbating diseases caused by them. Objectives: The present study was conducted to evaluates the presence of virulence factors and antibiotic susceptibility among Enterococcus faecalis isolated from sheep. Methods: The study included the collection of 50 samples (25 Milk samples collected from the udder was washed and the teats were disinfected and dried using alcohol, the first milk drop removed. 5ml of milk collected on aseptic tube and 25 Feces samples collected from sheep diarrhea from rectal by aseptic gloves. (from October 2018 to March 2019 ) and transported to laboratory as soon as possible in sterile Brain heart infusion broth that incubated at 37 C for at least 24-28 hours to increasing chances of isolation. Enterococcus faecalis that were recognized by cultural characteristics, Gram stain, and biochemical reactions. Results: The results of the laboratory cultural of 50 cotton swabs used s show that the isolation rate of Enterococcus spp. were 32% and 56% from milk and feaces respectively. the result of PCR test for detection of Enterococcus faecalis: show that the Enterococcus faecalis detected in rate of 66.6% from total Enterococcus spp. While the result of Enterococcus faecalis virulence factors showed that the Surface proteins, Gelatinase and Hemolysin were 75%, 33.3%, 25.5% respectively. Results of antibiotic sensitivity test showed the most bacterial isolated sensitive Nitrofurantoin , Imipenem and Nalidixic acid were 91.6%,83.3% and 58.3% % respectively Conclusion: We report that our simple modification of the existing multiplex PCR had increased the detection of the enterococcal virulence genes. Predominance of virulence genes was in order of Surface proteins, Gelatinase and Hemolysin were 75%, 33.3%, 25.5%. This modified PCR protocol could be useful to resolve the problem of decreased detection of virulence determinants in enterococci.