Online ISSN: 2515-8260

Keywords : Enterococcus faecalis


DETECTION of SOME VIRULENCE FACTORS and ANTIBIOTIC SENSITIVITY TEST of ENTEROCOCCUS FAECALIS ISOLATED FROM SHEEP by MULTIPLEX PCR

Hala Mohammed Majeed; Bashar Sadeq Noomi; Marwan Q. AL-Samarraie

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 68-74

Enterococcus faecalis form an important population of commensal bacteria and have been reported to possess numerous virulence factors considered significantly important in exacerbating diseases caused by them. Objectives: The present study was conducted to evaluates the presence of virulence factors and antibiotic susceptibility among Enterococcus faecalis isolated from sheep. Methods: The study included the collection of 50 samples (25 Milk samples collected from the udder was washed and the teats were disinfected and dried using alcohol, the first milk drop removed. 5ml of milk collected on aseptic tube and 25 Feces samples collected from sheep diarrhea from rectal by aseptic gloves. (from October 2018 to March 2019 ) and transported to laboratory as soon as possible in sterile Brain heart infusion broth that incubated at 37 C for at least 24-28 hours to increasing chances of isolation. Enterococcus faecalis that were recognized by cultural characteristics, Gram stain, and biochemical reactions. Results: The results of the laboratory cultural of 50 cotton swabs used s show that the isolation rate of Enterococcus spp. were 32% and 56% from milk and feaces respectively. the result of PCR test for detection of Enterococcus faecalis: show that the Enterococcus faecalis detected in rate of 66.6% from total Enterococcus spp. While the result of Enterococcus faecalis virulence factors showed that the Surface proteins, Gelatinase and Hemolysin were 75%, 33.3%, 25.5% respectively. Results of antibiotic sensitivity test showed the most bacterial isolated sensitive Nitrofurantoin , Imipenem and Nalidixic acid were 91.6%,83.3% and 58.3% % respectively Conclusion: We report that our simple modification of the existing multiplex PCR had increased the detection of the enterococcal virulence genes. Predominance of virulence genes was in order of Surface proteins, Gelatinase and Hemolysin were 75%, 33.3%, 25.5%. This modified PCR protocol could be useful to resolve the problem of decreased detection of virulence determinants in enterococci.

Comparative Evaluation Of Antimicrobial Efficacy Of Three Endodontic Irrigating Solutions Against Enterococcus Faecalis

Kanwalpreet Kaur Bhullar; Shubhneet Kaur; Shantun Malhotra; Harshdeep Singh; Aashish Handa; Rupam Kaur

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 8, Pages 4800-4804

Background: Irrigants and intracanal medicaments form a necessary adjunct that enhances the antimicrobial effect of mechanical cleansing and thus augments overall clinical efficacy. Hence; the present study was undertaken for analyzing and comparing the efficacy of three endodontic irrigating solutions against Enterococcus Faecalis.
Materials & methods: A total of thirty single rooted mandibular first premolar specimens were included. Sectioning of the specimens was done at cement-enamel junction level. Instrumentation of the root canals was done with rotary instruments to an apical size of ProTaper F3. Vertical sectioning of all the specimens was done along the mid-sagittal plane into two halves. All the specimens were divided into following study groups: Group A: 5% Sodium hypochlorite, Group B: 0.2% Chitosan and Group C: Saline. Inoculation of the bacterium was done in the broth in all the tissue culture wells. The biofilm on root canal surface was taken with a sterile scalpel and inoculated on agar plates and incubated. Colony forming units were then analyzed. Analysis of all the results was done using SPSS software.
Results: Mean CFU among specimens of group A, group B and group C were found to be 0.59, 2.118 and 90.184 respectively. While analyzing the results statistically, significant results were obtained.
Conclusion: Sodium hypochlorite exhibited maximum efficacy against Enterococcus Faecalis.